Fujita Naoyuki, Wade Paul A
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Whitehead Building, Room 142, 615 Michael Street, Atlanta, GA 30322, USA.
Methods. 2004 May;33(1):81-5. doi: 10.1016/j.ymeth.2003.10.022.
Chromatin remodeling complexes consist of multiple subunits, some of which are in intimate contact with DNA while others are not. The ability to effectively cross-link proteins to DNA with formaldehyde is impacted by the average distance between the two species. Productive cross-linking of proteins not in direct contact with DNA is greatly facilitated by the inclusion of an initial cross-linking reaction using bifunctional imidoester cross-linking reagents.
染色质重塑复合物由多个亚基组成，其中一些亚基与DNA紧密接触，而另一些则不然。用甲醛有效地将蛋白质与DNA交联的能力受这两种物质之间平均距离的影响。使用双功能亚胺酯交联试剂进行初始交联反应，极大地促进了与DNA没有直接接触的蛋白质的有效交联。
