Montoro P, Carbone V, Quiroz J de Dioz Zuniga, De Simone F, Pizza C
Dipartimento di Scienze Farmaceutiche, Facoltá di Farmacia, Universitá di Salerno, Via Ponte don Melillo, 84084 Fisciano, Italy.
Phytochem Anal. 2004 Jan-Feb;15(1):55-64. doi: 10.1002/pca.740.
The two main classes of secondary metabolites, alkaloids and quinovic acid glycosides, of Uncaria tomentosa (Willd.) DC. (Rubiaceae), a Peruvian plant commonly known as 'uña de gato', have been analysed. Separation of the alkaloidal fraction was achieved using a solid phase extraction method based on cationic exchange, and an analytical method employing HPLC-ES/MS has been developed. Quantitative data for commercial wild bark, cultivated bark and leaves are reported. The analysis of quinovic acid glycosides was performed directly on the crude extract using both a fast analytical method based on flow injection ES/MS, and a more complete analytical technique using HPLC-MS.
对秘鲁一种俗称“猫爪”的植物——毛钩藤（茜草科）的两类主要次生代谢产物生物碱和奎诺酸糖苷进行了分析。采用基于阳离子交换的固相萃取方法实现了生物碱部分的分离，并开发了一种采用高效液相色谱 - 电喷雾质谱联用的分析方法。报告了市售野生树皮、栽培树皮和树叶的定量数据。采用基于流动注射电喷雾质谱联用的快速分析方法以及使用高效液相色谱 - 质谱联用的更完整分析技术，直接对粗提物中的奎诺酸糖苷进行了分析。
