Fonseca Fabiana N, Tavares Marina F M
Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, São Paulo-SP, 05508-900, Brazil.
Phytochem Anal. 2004 Jan-Feb;15(1):65-70. doi: 10.1002/pca.744.
A capillary electrophoretic method for the quantification of free and total apigenin in methanolic, ethanolic and glycolic extracts of Chamomilla recutita L. Rauschert (Asteraceae) is described. The method was validated for measurement of apigenin in the range 5.00-300 microg/mL (r2 = 0.993) and showed coefficients of intra-day (replicability) and inter-day (repeatability) variability of better than 2%. The limits of detection and quantification were 3.80 and 11.5 microg/mL, respectively, and the average recovery was 102.0 +/- 0.8% at three concentration levels of apigenin. Free and total apigenin contents in the extracts were, respectively, determined as 106 and 903 microg/g (methanolic extract), 77 and 817 microg/g (ethanolic extract) and 11.0 and 247 microg/g (glycolic extract).
本文描述了一种毛细管电泳法，用于定量测定德国洋甘菊（菊科）甲醇提取物、乙醇提取物和乙醇酸提取物中的游离芹菜素和总芹菜素。该方法在5.00-300μg/mL范围内测定芹菜素时得到验证（r2 = 0.993），日内（重复性）和日间（重现性）变异系数均优于2%。检测限和定量限分别为3.80和11.5μg/mL，在芹菜素的三个浓度水平下平均回收率为102.0±0.8%。提取物中游离芹菜素和总芹菜素的含量分别为106和903μg/g（甲醇提取物）、77和817μg/g（乙醇提取物）以及11.0和247μg/g（乙醇酸提取物）。
