Whitaker Thomas B, Richard John L, Giesbrecht Francis G, Slate Andrew B, Ruiz Nelson
US Department of Agriculture, Agricultural Research Service, PO Box 7625, North Carolina State University, Raleigh, NC 27695-7625, USA.
J AOAC Int. 2003 Nov-Dec;86(6):1187-92.
To determine if deoxynivalenol (DON) is concentrated in small corn screenings, fourteen to twenty-three 1.1 kg test samples were taken from each of 10 barges of shelled corn. Each of the 181 test samples was divided into 2 components (fines and clean) using a 5 mm screen. The clean component sample rode the 5 mm screen and the fines component sample passed through the 5 mm screen. The DON concentration in fines component sample was about 3 times the DON concentration in the clean component sample. The DON in the 181 fines and clean component samples averaged 689.0 and 206.1 ng/g, respectively. Regression equations were developed to predict the DON in the barge based upon measurements of DON in the fines component sample. The ratio of DON in the lot to DON in the fines component sample was 0.359. The coefficient of variation (CV) associated with predicting the DON concentration in a lot with 359 ng/g using a 1.1 kg test sample was 47.0%. Increasing sample size to 4.4 kg reduced the CV to 23%.
为了确定脱氧雪腐镰刀菌烯醇（DON）是否在小颗粒玉米筛出物中富集，从10艘脱壳玉米驳船中的每一艘采集了14至23个1.1千克的测试样品。使用5毫米筛网将181个测试样品中的每一个都分成两个部分（细粉和净粮）。净粮部分样品留在5毫米筛网上，细粉部分样品通过5毫米筛网。细粉部分样品中的DON浓度约为净粮部分样品中DON浓度的3倍。181个细粉和净粮部分样品中的DON平均含量分别为689.0纳克/克和206.1纳克/克。根据细粉部分样品中DON的测量值建立了回归方程，以预测驳船中的DON含量水平。批次中DON与细粉部分样品中DON的比例为0.359。使用1.1千克测试样品预测批次中DON浓度为359纳克/克时的变异系数（CV）为47.0%。将样品量增加到4.4千克可将CV降低到23%。
