Isenburg Jason C, Simionescu Dan T, Vyavahare Naren R
501 Rhodes Research Centre, Department of Bioengineering, Clemson University, Clemson, SC 29634, USA.
Biomaterials. 2004 Jul;25(16):3293-302. doi: 10.1016/j.biomaterials.2003.10.001.
The long-term performance of tissue-derived, glutaraldehyde (Glut)-treated cardiovascular implants such as prosthetic heart valves and vascular grafts is limited by the bio-degeneration of tissue components. While collagen is satisfactorily preserved by Glut, elastin is not stabilized and is highly vulnerable to degradation. The aim of our studies was to develop methods for efficient stabilization of elastin and subsequently reduce its vulnerability towards enzymatic degradation. More specifically, we investigated the use of tannic acid (TA)1 as a novel agent that specifically targets elastin stabilization. Basic investigations on in vitro interactions between Glut, TA and pure aortic elastin provided clear evidence that Glut treatment does not protect elastin from enzymatic degradation. TA bound to elastin in a time-dependent pattern and this binding increased the resistance of elastin to enzymatic degradation. In addition, when TA was used in mixture with Glut, the kinetic of TA binding to elastin was enhanced and this was translated into improved elastin stabilization. Our results clearly documented the superiority of TA as an elastin-stabilizing agent by comparison with the commonly utilized Glut-based tissue crosslinking techniques.
组织来源的、经戊二醛（Glut）处理的心血管植入物（如人工心脏瓣膜和血管移植物）的长期性能受到组织成分生物降解的限制。虽然胶原蛋白能被戊二醛较好地保存，但弹性蛋白未得到稳定，极易降解。我们研究的目的是开发有效稳定弹性蛋白的方法，进而降低其对酶促降解的易感性。更具体地说，我们研究了使用鞣酸（TA）作为一种专门针对弹性蛋白稳定化的新型试剂。对戊二醛、鞣酸与纯主动脉弹性蛋白之间体外相互作用的基础研究提供了明确证据，表明戊二醛处理并不能保护弹性蛋白免受酶促降解。鞣酸以时间依赖性方式与弹性蛋白结合，这种结合增加了弹性蛋白对酶促降解的抗性。此外，当鞣酸与戊二醛混合使用时，鞣酸与弹性蛋白结合的动力学增强，这转化为弹性蛋白稳定性的提高。与常用的基于戊二醛的组织交联技术相比，我们的结果清楚地证明了鞣酸作为弹性蛋白稳定化试剂的优越性。
