Zhu Xiaofeng, Kamande Mary W, Thiam Serigne, Kapnissi Constantina P, Mwongela Simon M, Warner Isiah M
Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.
Electrophoresis. 2004 Feb;25(4-5):562-8. doi: 10.1002/elps.200305713.
We describe the use of the polymeric surfactant poly(sodium undecylenic sulfate) (poly-SUS) as a stationary phase coating in open-tubular capillary electrochromatography (OT-CEC) coupled with electrospray ionization-mass spectrometry (ESI-MS) for the analysis of beta-blocker and benzodiazepine analytes. The production of a polymeric surfactant coating on the capillary inner wall involves (i) adsorption of the cationic polymer poly(diallyldimethylammonium chloride) (PDADMAC) to the inner surface of capillary, and (ii) adsorption of the negatively charged poly-SUS onto the cationic polymer layer via strong physical interaction of the two polymer layers. As compared with micellar electrokinetic chromatography (MEKC) coupled with ESI-MS, the main advantage of this proposed method is minimization of introduction of the monomeric or polymeric surfactant into the mass spectrometer, thus avoiding the interference of the nonvolatile micelle in ESI-MS. The effects of buffer pH and applied voltage on the separation of the analytes are also discussed. Under optimum conditions, four of the five beta-blockers and four benzodiazepines are separated.
我们描述了将聚合表面活性剂聚（十一烷基硫酸钠）（聚-SUS）用作开管毛细管电色谱（OT-CEC）中的固定相涂层，并与电喷雾电离质谱（ESI-MS）联用，用于分析β受体阻滞剂和苯二氮䓬类分析物。在毛细管内壁上形成聚合表面活性剂涂层的过程包括：（i）阳离子聚合物聚（二烯丙基二甲基氯化铵）（PDADMAC）吸附到毛细管内表面，以及（ii）通过两个聚合物层之间的强物理相互作用，将带负电荷的聚-SUS吸附到阳离子聚合物层上。与联用ESI-MS的胶束电动色谱（MEKC）相比，该方法的主要优点是将单体或聚合表面活性剂引入质谱仪的量减至最少，从而避免了ESI-MS中非挥发性胶束的干扰。还讨论了缓冲液pH值和施加电压对分析物分离的影响。在最佳条件下，五种β受体阻滞剂中的四种和四种苯二氮䓬类药物得以分离。
