Lu W, Shamsi S A, McCarley T D, Warner I M
Department of Chemistry, Louisiana State University, Baton Rouge 70803, USA.
Electrophoresis. 1998 Sep;19(12):2193-9. doi: 10.1002/elps.1150191225.
On-line capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) has been used to determine the tricyclic antidepressant drugs (imipramine, doxepin, and amitriptyline) as well as the beta-adrenergic blocker drugs (propranolol and alprenolol). A CE-ESI-MS interface linking a manually operated CE system and a Finnigan MAT-900 sector mass spectrometer (with an Analytica electrospray ionization source) has been constructed in-house and employed for this study. Although a water/methanol based capillary zone electrophoresis (CZE) buffer was initially used to determine these analytes, enhanced resolution was obtained by addition of a polymerized surfactant, i.e., poly-sodium undecylenic sulfate (poly-SUS), into the electrokinetic chromatography (EKC) buffer. When a low concentration of this poly-SUS surfactant was added to a volatile EKC buffer, these structurally similar cationic drugs were EKC separated and on-line detected by ESI-MS.
在线毛细管电泳-电喷雾电离-质谱联用技术（CE-ESI-MS）已被用于测定三环类抗抑郁药物（丙咪嗪、多塞平、阿米替林）以及β-肾上腺素能阻滞剂药物（普萘洛尔和阿普洛尔）。本研究中，我们自行构建了一个连接手动操作的CE系统和Finnigan MAT-900扇形质谱仪（配备Analytica电喷雾电离源）的CE-ESI-MS接口。尽管最初使用基于水/甲醇的毛细管区带电泳（CZE）缓冲液来测定这些分析物，但通过在电动色谱（EKC）缓冲液中添加聚合表面活性剂，即十一烯酸钠聚合物（poly-SUS），分辨率得到了提高。当向挥发性EKC缓冲液中添加低浓度的这种poly-SUS表面活性剂时，这些结构相似的阳离子药物在EKC中得以分离，并通过ESI-MS进行在线检测。
