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采用水蒸馏法、热解吸法和顶空气相色谱/质谱联用技术分析百里香薄荷精油及其抗菌活性。

Analysis of essential oil of Satureja thymbra by hydrodistillation, thermal desorber, and headspace GC/MS techniques and its antimicrobial activity.

作者信息

Gören Ahmet C, Topçu Gülaçti, Bilsel Gökhan, Bilsel Mine, Wilkinson Jenny M, Cavanagh Heather

机构信息

Department of Chemistry, TUBITAK, UME, P.O. Box 54, Gebze, Kocaeli, Turkey.

出版信息

Nat Prod Res. 2004 Apr;18(2):189-95. doi: 10.1080/14786410310001608145.

Abstract

The essential oil composition of Satureja thymbra was analyzed by direct thermal desorber and Headspace GC/MS analysis methods. Its constituents were determined to be mainly carvacrol (40.15%), gamma-terpinene (26.56%), p-cymene (16.39%), and thymol (13.16%). The other techniques, thermal desorber and Headspace GC/MS, were used for the plant leaves at three different temperature, which showed similar results. The thermal desorber GC/MS gave better and more sensitive results than Headspace GC/MS. The essential oil was found to be active against the bacteria Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella sonnei, and Staphylococcus aureus and the yeast Candida albicans.

摘要

采用直接热脱附仪和顶空气相色谱/质谱联用分析法对百里香薄荷的精油成分进行了分析。其成分主要确定为香芹酚(40.15%)、γ-萜品烯(26.56%)、对伞花烃(16.39%)和百里香酚(13.16%)。另外,热脱附仪和顶空气相色谱/质谱联用技术用于三种不同温度下的植物叶片分析,结果相似。热脱附气相色谱/质谱联用仪比顶空气相色谱/质谱联用仪给出的结果更好、更灵敏。发现该精油对大肠杆菌、铜绿假单胞菌、鼠伤寒沙门氏菌、宋内志贺氏菌、金黄色葡萄球菌等细菌以及白色念珠菌具有活性。

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