Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P6028820, Palestine.
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus P6028820, Palestine.
Molecules. 2021 Dec 26;27(1):125. doi: 10.3390/molecules27010125.
(Danin and Hedge) Bräuchler is a perennial herb in the Lamiaceae family that was discovered and classified in 1998. This green herb is restricted to the mountains overlooking the Dead Sea, specifically in Jordan's southwest, the Edom mountains, and the Tubas mountains in Palestine. Gas chromatography-mass spectrometry (GC-MS) analysis of essential oil (EO) of air-dried and fresh resulted in the identification of 30 and 42 phytochemicals accounting for 99.56 and 98.64% of the EO, respectively. Thymol (46.07 ± 1.1 and 40.64 ± 1.21%) was the major compound, followed by its biosynthetic precursors -terpinene (21.15 ± 1.05% and 20.65 ± 1.12%), and -cymene (15.02 ± 1.02% and 11.51 ± 0.97%), respectively. Microdilution assay was used to evaluate the antimicrobial property of EOs against (ATCC 25923), clinical isolate Methicillin-Resistant (MRSA), (ATCC 700221) (ATCC 13883), (ATCC 700221), (ATCC 25922) and (ATCC 27853) and (ATCC-90028). With a MIC of 0.135 μg/mL, the EOs has the most potent antibacterial action against . Both EOs display good antifungal efficacy against , with a MIC value of 0.75 μg/mL, which was better than that of Fluconazole's (positive control, MIC = 1.56 μg/mL). The antioxidant capacity of EOs extracted from air-dried and fresh was determined using the DPPH assay, with IC values of 4.78 ± 0.41 and 5.37 ± 0.40 μg/mL, respectively. The tested EOs showed significant cytotoxicity against Hela, HepG2, and COLO-205 cells, with IC values ranging from 82 ± 0.98 to 256 ± 1.95 μg/mL. The current work shows there is a possibility to use the EOs for various applications.
(Danin 和 Hedge) Bräuchler 是唇形科的一种多年生草本植物，于 1998 年被发现并分类。这种绿色草本植物仅生长在俯瞰死海的山脉中，具体来说，分布在约旦西南部的以土巴山区和巴勒斯坦的图巴斯山区。对风干和新鲜的植物精油（EO）进行气相色谱-质谱（GC-MS）分析，鉴定出分别占 EO 99.56%和 98.64%的 30 种和 42 种植物化学物质。百里香酚（46.07±1.1 和 40.64±1.21%）是主要化合物，其次是其生物合成前体 -萜品烯（21.15±1.05%和 20.65±1.12%）和 -柠檬烯（15.02±1.02%和 11.51±0.97%）。微量稀释法评估 EO 对 （ATCC 25923）、临床分离的耐甲氧西林金黄色葡萄球菌（MRSA）、 （ATCC 700221） （ATCC 13883）、 （ATCC 700221）、 （ATCC 25922）和 （ATCC 27853）以及 （ATCC-90028）的抗菌特性。EOs 的 MIC 值为 0.135 μg/mL，对 具有最强的抗菌作用。两种 EOs 对 显示出良好的抗真菌作用，MIC 值为 0.75 μg/mL，优于氟康唑（阳性对照，MIC=1.56 μg/mL）。使用 DPPH 法测定风干和新鲜 Bräuchler 提取物的抗氧化能力，其 IC 值分别为 4.78±0.41 和 5.37±0.40 μg/mL。测试的 EOs 对 Hela、HepG2 和 COLO-205 细胞表现出显著的细胞毒性，IC 值范围为 82±0.98 至 256±1.95 μg/mL。目前的工作表明，Bräuchler 植物精油有可能用于各种应用。
