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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Gabriel J E, Ferro J A, Stefani R M, Ferro M I, Gomes S L, Macari M

Departamento de Tecnologia, Faculdade de Ciencias Agrárias e Veterinárias, Universidade Estadual Paulista-UNESP, Jaboticabal, Brazil.

Br Poult Sci. 1996 May;37(2):443-9. doi: 10.1080/00071669608417875.

The synthesis of heat shock protein 70 (Hsp70) mRNA and the expression of Hsp70 in the liver of broiler chickens submitted to acute heat stress (35 degrees C for 5 h) was investigated. 2. Hsp70 expression was detected by SDS-PAGE and Western blot analysis using a polyclonal antiserum against Hsp70 of Blastocladiella emersonii. The specific signal of Hsp70 mRNA was analysed by Northern blot using as probe a Hsp70 cDNA of B. emersonii. 3. An increase in the amount of Hsp70 was detected from the first up to the fifth hour of acute heat exposure. This increase in the amount of Hsp70 was accompanied by an increase in Hsp70 mRNA which peaked at 3 h. 4. This study shows that the heat induced increase in Hsp70 mRNA and protein in broiler liver, in vivo, are time dependent, similar to that in mammals.

研究了急性热应激（35℃，5小时）下肉鸡肝脏中热休克蛋白70（Hsp70）mRNA的合成及Hsp70的表达。2. 通过SDS-PAGE和使用针对艾美球虫Hsp70的多克隆抗血清进行蛋白质免疫印迹分析检测Hsp70表达。使用艾美球虫的Hsp70 cDNA作为探针，通过Northern印迹分析Hsp70 mRNA的特异性信号。3. 在急性热暴露的第1小时到第5小时检测到Hsp70量增加。Hsp70量的这种增加伴随着在3小时达到峰值的Hsp70 mRNA的增加。4. 本研究表明，热诱导的肉鸡肝脏中Hsp70 mRNA和蛋白质的增加在体内是时间依赖性的，与哺乳动物中的情况相似。

Gabriel J E, Ferro J A, Stefani R M, Ferro M I, Gomes S L, Macari M

Departamento de Tecnologia, Faculdade de Ciencias Agrárias e Veterinárias, Universidade Estadual Paulista-UNESP, Jaboticabal, Brazil.

Br Poult Sci. 1996 May;37(2):443-9. doi: 10.1080/00071669608417875.

The synthesis of heat shock protein 70 (Hsp70) mRNA and the expression of Hsp70 in the liver of broiler chickens submitted to acute heat stress (35 degrees C for 5 h) was investigated. 2. Hsp70 expression was detected by SDS-PAGE and Western blot analysis using a polyclonal antiserum against Hsp70 of Blastocladiella emersonii. The specific signal of Hsp70 mRNA was analysed by Northern blot using as probe a Hsp70 cDNA of B. emersonii. 3. An increase in the amount of Hsp70 was detected from the first up to the fifth hour of acute heat exposure. This increase in the amount of Hsp70 was accompanied by an increase in Hsp70 mRNA which peaked at 3 h. 4. This study shows that the heat induced increase in Hsp70 mRNA and protein in broiler liver, in vivo, are time dependent, similar to that in mammals.

研究了急性热应激（35℃，5小时）下肉鸡肝脏中热休克蛋白70（Hsp70）mRNA的合成及Hsp70的表达。2. 通过SDS-PAGE和使用针对艾美球虫Hsp70的多克隆抗血清进行蛋白质免疫印迹分析检测Hsp70表达。使用艾美球虫的Hsp70 cDNA作为探针，通过Northern印迹分析Hsp70 mRNA的特异性信号。3. 在急性热暴露的第1小时到第5小时检测到Hsp70量增加。Hsp70量的这种增加伴随着在3小时达到峰值的Hsp70 mRNA的增加。4. 本研究表明，热诱导的肉鸡肝脏中Hsp70 mRNA和蛋白质的增加在体内是时间依赖性的，与哺乳动物中的情况相似。