热应激肉鸡中热休克蛋白90（Hsp90）的表达及其相应mRNA的转录

Expression of heat shock protein 90 (Hsp90) and transcription of its corresponding mRNA in broilers exposed to high temperature.

Lei Lei, Yu Jimian, Bao Endong

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.

Br Poult Sci. 2009 Jul;50(4):504-11. doi: 10.1080/00071660903110851.

Abstract

In order to understand the distribution and variation of Hsp90 and its corresponding mRNA in the tissues of heat-stressed broilers, 100 experimental birds were heat stressed for 0, 2, 3, 5 and 10 h. 2. Both nuclear and cytoplasmic Hsp90-positive signals were observed in myocardial cells, hepatocytes and renal tubule epithelial cells. The expression of Hsp90 increased in the heart, liver and kidney of broilers after exposure to high temperature for 2 h. 3. Following non-significant reduction after 3 and 5 h in the treatment groups, the expression of Hsp90 in the heart and kidney, but not in the liver, increased again after 10 h of heat stress. In the heart and kidney, hsp90 mRNA transcription levels exhibited the same trend as the protein expression of Hsp90. 4. Induction of hsp90 mRNA and Hsp90 protein at an early stressing stage indicated that heat stress can directly stimulate and quickly initiate the transcription of hsp90 mRNA and translation of Hsp90 protein to protect cells. 5. Hsp90 was strongly expressed in endothelial cells and blood vessel walls, suggesting that there is a relationship between vasoconstriction and Hsp90 localisation, which influences blood flow regulation.

摘要

为了解热应激肉鸡组织中热休克蛋白90（Hsp90）及其相应信使核糖核酸（mRNA）的分布与变化，对100只实验鸡分别进行0、2、3、5和10小时的热应激处理。2. 在心肌细胞、肝细胞和肾小管上皮细胞中均观察到细胞核和细胞质中Hsp90阳性信号。高温处理2小时后，肉鸡心脏、肝脏和肾脏中Hsp90的表达增加。3. 处理组在3小时和5小时后Hsp90表达无显著降低，热应激10小时后，心脏和肾脏中Hsp90的表达再次增加，但肝脏中未增加。在心脏和肾脏中，hsp90 mRNA转录水平与Hsp90蛋白表达呈现相同趋势。4. 在应激早期hsp90 mRNA和Hsp90蛋白的诱导表明，热应激可直接刺激并快速启动hsp90 mRNA的转录和Hsp90蛋白的翻译以保护细胞。5. Hsp90在内皮细胞和血管壁中强烈表达，提示血管收缩与Hsp90定位之间存在关联，这会影响血流调节。