Tsuchiya A, Nihei M, Ando Y, Suzuki S, Kimijima I, Abe R
Second Department of Surgery, Fukushima Medical College, Japan.
Surg Today. 1992;22(2):105-9. doi: 10.1007/BF00311332.
The relationship of estrogen receptor (ER) status to DNA ploidy was investigated in 121 patients with breast cancer who underwent surgery. Lymph node status was evaluated histologically and ER levels were determined by the dextran-coated charcoal method, with a level of 3 fmol/mg.protein being considered positive. Flow cytometric DNA content was analyzed using paraffin-embedded tissue blocks. Sixty-three percent of the specimens were ER+, while 37 percent were negative. Sixty-one patients (50.4 percent) were diploid and 60 aneuploid. A statistically significant correlation between the ER positivity rate and diploid DNA was found. Higher ER levels were seen in the postmenopausal patients with diploid tumors than in those with aneuploid tumors and there was a significant tendency for ER levels to be higher in the diploid tumors. Nodal status was not correlated with ER positivity or ploidy pattern. The present results indicate that ER levels are correlated with DNA ploidy, and reflect the degree of functional differentiation.
对121例行手术治疗的乳腺癌患者，研究了雌激素受体（ER）状态与DNA倍体的关系。通过组织学评估淋巴结状态，采用葡聚糖包被活性炭法测定ER水平，以3 fmol/mg蛋白质的水平为阳性。使用石蜡包埋组织块分析流式细胞术DNA含量。63%的标本ER阳性，37%为阴性。61例患者（50.4%）为二倍体，60例为非整倍体。发现ER阳性率与二倍体DNA之间存在统计学显著相关性。绝经后二倍体肿瘤患者的ER水平高于非整倍体肿瘤患者，且二倍体肿瘤中ER水平有显著升高趋势。淋巴结状态与ER阳性或倍体模式无关。目前的结果表明，ER水平与DNA倍体相关，并反映功能分化程度。
