Middleton N, Jelen P, Bell G
Faculty of Agriculture, Food and Nutritional Sciences, University of Alberta, Edmonton, Alberta, Canada.
Int J Food Sci Nutr. 2004 Mar;55(2):131-41. doi: 10.1080/096374080410001666504.
Sedentary male subjects (n=9) on a controlled diet were fed two doses (0.8 or 1.6 g/kg body mass) of a whey protein isolate (WPI), in addition to an isocaloric placebo; blood samples were drawn over a 4-h period and glutathione concentration determined. There was no effect of the supplementation at either level over the 4-h sampling period. The effects of a WPI supplemented diet on glutathione concentrations in whole blood as well as peripheral mononuclear cell populations were also investigated over a 6-week period in male subjects (n=18) involved in arduous aerobic training; blood was collected prior to and following a 40 km simulated cycling trial. The aerobic training period resulted in significantly lower glutathione concentrations in whole blood, an effect that was mitigated by WPI supplementation. A significant increase in mononuclear cell glutathione was also observed in subjects receiving the WPI supplement following the 40 km simulated cycling trial.
对9名遵循控制饮食的久坐男性受试者，除给予等热量安慰剂外，还喂食两剂（0.8或1.6克/千克体重）乳清蛋白分离物（WPI）；在4小时内采集血样并测定谷胱甘肽浓度。在4小时采样期内，任一水平的补充剂均无效果。在为期6周的时间里，还对18名参与艰苦有氧训练的男性受试者，研究了补充WPI的饮食对全血谷胱甘肽浓度以及外周单核细胞群体的影响；在40公里模拟自行车试验前后采集血样。有氧训练期导致全血谷胱甘肽浓度显著降低，而WPI补充可减轻这种影响。在40公里模拟自行车试验后，接受WPI补充剂的受试者单核细胞谷胱甘肽也显著增加。
