Wimmer A, Graf A, Wank R
Institute of Immunology, University of Munich, FRG.
Infusionsther Transfusionsmed. 1992 Jun;19(3):130-3. doi: 10.1159/000222602.
The key role of HLA polymorphism in the pathogenesis of many diseases and in determining compatibility in transplantation makes it desirable to employ automated systems for identifying HLA polymorphism. For more than one year we have used the photometer system Patimed commercially available from the Leica company. We compared the results of the conventional cell-mediated lympholysis test and conventional serological HLA typing with the results obtained by the Patimed system. Despite some good preliminary results it would be premature, in our opinion, to recommend the Patimed system for routine assessment of killer cell activity whereas we can definitely recommend the Patimed system to define HLA class I and class II molecules serologically for research or routine purposes.
HLA多态性在许多疾病的发病机制以及确定移植兼容性方面发挥着关键作用，这使得采用自动化系统来识别HLA多态性成为必要。一年多来，我们一直在使用徕卡公司市售的Patimed光度计系统。我们将传统的细胞介导淋巴细胞溶解试验和传统血清学HLA分型结果与Patimed系统获得的结果进行了比较。尽管取得了一些良好的初步结果，但我们认为，推荐Patimed系统用于常规评估杀伤细胞活性还为时过早，而我们绝对可以推荐Patimed系统用于在研究或常规目的中血清学鉴定HLA I类和II类分子。
