Peterson Kenneth R
Department of Biochemistry and Molecular Biology, 3901 Rainbow Blvd, University of Kansas Medical Center, Kansas City, KS 66160, USA.
Expert Rev Mol Med. 2003 Apr 7;5(13):1-25. doi: 10.1017/S1462399403006082.
The generation of transgenic mice with yeast artificial chromosomes (YACs) has proven to be a valuable system to: (1) study gene structure-function relationships; (2) produce mouse models of human disease; (3) complement mouse mutants; (4) generate mice bioreactors; and (5) screen YAC libraries in vivo. Continued refinement of current techniques and development of new protocols should encourage widespread adaptation of this strategy for these and other applications. Use of whole loci as transgenes is an important improvement in murine transgenesis because it results in a more realistic pattern and level of gene expression during ontogeny. Application of this technology to develop human artificial chromosomes (HACs) might provide the next generation of gene therapy vectors that will overcome most of the problems and barriers associated with current vector systems.
事实证明，利用酵母人工染色体（YAC）生成转基因小鼠是一个有价值的系统，可用于：（1）研究基因结构与功能的关系；（2）建立人类疾病的小鼠模型；（3）补充小鼠突变体；（4）培育小鼠生物反应器；以及（5）在体内筛选YAC文库。对现有技术的不断完善和新方案的开发应会促使这种策略在这些及其他应用中得到更广泛的采用。将整个基因座用作转基因是小鼠转基因技术的一项重要改进，因为它能在个体发育过程中产生更符合实际的基因表达模式和水平。将该技术应用于开发人类人工染色体（HAC）可能会提供下一代基因治疗载体，从而克服与当前载体系统相关的大多数问题和障碍。
