Lamb B T, Gearhart J D
Department of Gynecology and Obsterics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287-2501, USA.
Curr Opin Genet Dev. 1995 Jun;5(3):342-8. doi: 10.1016/0959-437x(95)80049-2.
Advances in yeast artificial chromosome (YAC) technologies over the past decade have enabled the precise identification and manipulation of large genomic regions (>100 kb) of DNA. Introduction of YACs into the mouse germline has now been accomplished through transfection of mouse embryonic stem cells as well as through pronuclear microinjection, allowing the efficient transfer defined genomic loci into mice. YAC transgenics will have a profound impact on the development of transgenic mice as bioreactors and as models of human disease, and on the functional analysis of higher order genomic structure.
在过去十年中，酵母人工染色体（YAC）技术取得的进展使得对大于100 kb的大型基因组区域进行精确鉴定和操作成为可能。目前，通过对小鼠胚胎干细胞进行转染以及原核显微注射，已成功将YAC导入小鼠种系，从而能够将特定的基因组位点高效导入小鼠体内。YAC转基因技术将对作为生物反应器和人类疾病模型的转基因小鼠的开发，以及对高阶基因组结构的功能分析产生深远影响。
