[噪声磁场阻断工频磁场与佛波酯诱导的共抑制效应]

[Noise magnetic fields block co-suppression effect induced by power frequency magnetic field and phorbol ester].

作者信息

Gao Xiang-wei, Xu Zheng-ping, Huo Ya-nan, Jiang Huai, Fu Yi-ti, Lu De-qiang, Zeng Qun-li

机构信息

Zhejiang Provincial Key Laboratory of Bioelectromagnetics, Zhejiang University School of Medicine, Hangzhou 310031, China.

出版信息

Zhonghua Yu Fang Yi Xue Za Zhi. 2004 Jan;38(1):11-3.

PMID:14989891

Abstract

OBJECTIVES

To explore intervention with electromagnetic noise for co-suppression effect on gap-junctional intercellular communication (GJIC) induced or strengthened by low intensity magnetic field with carcinogen 12-O-tetradecanoylphorbol-13-acetate (TPA).

METHODS

Fibroblast cells from NIH 3T3 mice were exposed to extremely low intensity magnetic field (MF) 0.2 mT, 0.2 mT + TPA or/and electromagnetic noise with the same intensity of MF for 24 h, and GJIC was determined using fluorescence recovery analysis after photobleaching (FRAP) with a laser-scanning confocal microscope (Leica, Germany).

RESULTS

GJIC function could be co-suppressed by MF of 0.2 mT with TPA, with fluorescence recovery of (23 +/- 11)%, lower than that in the control group [(46 +/- 19)%] and in the group with TPA only [(34 +/- 17) %] (P < 0.01), indicating 0.2 mT MF plus TPA could co-inhibit GJIC (P < 0.01). Superposition of 0.2 mT noise MF could get a fluorescence recovery of (35 +/- 19)% and significantly antagonize its co-suppression by TPA.

CONCLUSION

Electromagnetic noise of 0.2 mT could block the intensifying effect of power frequency magnetic field on TPA-induced GJIC inhibition.

摘要

目的

探讨电磁噪声干预对低强度磁场与致癌物12-氧-十四烷酰佛波醇-13-乙酸酯（TPA）诱导或增强的间隙连接细胞间通讯（GJIC）的共抑制作用。

方法

将来自NIH 3T3小鼠的成纤维细胞暴露于0.2 mT的极低频磁场（MF）、0.2 mT + TPA或/和与MF强度相同的电磁噪声中24小时，使用激光扫描共聚焦显微镜（德国徕卡）通过光漂白后荧光恢复分析（FRAP）测定GJIC。

结果

0.2 mT的MF与TPA可共同抑制GJIC功能，荧光恢复率为（23±11）%，低于对照组[（46±19）%]和仅使用TPA的组[（34±17）%]（P < 0.01），表明0.2 mT MF加TPA可共同抑制GJIC（P < 0.01）。叠加0.2 mT噪声MF可使荧光恢复率达到（35±19）%，并显著拮抗TPA对其的共抑制作用。