Arora Charanjit, Kee Kehkooi, Maleki Shohreh, Keeney Scott
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center and Weill Graduate School of Medical Sciences of Cornell University, 1275 York Avenue, Box 97, New York, NY 10021, USA.
Mol Cell. 2004 Feb 27;13(4):549-59. doi: 10.1016/s1097-2765(04)00063-2.
Meiotic recombination initiates with double-strand breaks (DSBs) catalyzed by Spo11 in conjunction with accessory proteins whose roles are not understood. Two-hybrid analysis reveals a network of interactions connecting the yeast DSB proteins to one another. Of these proteins, Ski8 was known to function in cytoplasmic RNA metabolism, suggesting that its role in recombination might be indirect. However, obligate partners of Ski8 in RNA metabolism are dispensable for recombination and Ski8 relocalizes to the nucleus and associates with chromosomes specifically during meiosis. Interaction of Ski8 with Spo11 is essential for DSB formation and Ski8 relocalization. Thus, Ski8 plays distinct roles in RNA metabolism and, as a direct partner of Spo11, in DSB formation. Ski8 works with Spo11 to recruit other DSB proteins to meiotic chromosomes, implicating Ski8 as a scaffold protein mediating assembly of a multiprotein complex essential for DSB formation.
减数分裂重组起始于由Spo11催化的双链断裂(DSB),该过程还伴随着一些功能尚不明确的辅助蛋白。双杂交分析揭示了一个将酵母DSB蛋白相互连接起来的相互作用网络。在这些蛋白中,Ski8已知在细胞质RNA代谢中发挥作用,这表明它在重组中的作用可能是间接的。然而,Ski8在RNA代谢中的必需伙伴对于重组来说是可有可无的,并且Ski8会重新定位到细胞核,并在减数分裂期间特异性地与染色体结合。Ski8与Spo11的相互作用对于DSB形成和Ski8重新定位至关重要。因此,Ski8在RNA代谢中发挥着不同的作用,并且作为Spo11的直接伙伴,在DSB形成中也发挥作用。Ski8与Spo11协同作用,将其他DSB蛋白招募到减数分裂染色体上,这表明Ski8是一种支架蛋白,介导了对DSB形成至关重要的多蛋白复合物的组装。