Jolivet Sylvie, Vezon Daniel, Froger Nicole, Mercier Raphaël
Station de Génétique et d'Amélioration des Plantes, Institut Jean-Pierre Bourgin, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles cedex, France.
Genes Cells. 2006 Jun;11(6):615-22. doi: 10.1111/j.1365-2443.2006.00972.x.
Meiotic recombination involves the formation and repair of DNA double-strand breaks (DSB). One of the genes required for DSB formation in the yeast Saccharomyces cerevisiae, Ski8/Rec103, is intriguing because it also plays a role in cytoplasmic RNA metabolism, a function difficult to relate to DSB formation. The meiotic role of Ski8 is conserved in several fungi, but has not been investigated outside this kingdom. We identified the Ski8 homolog in Arabidopsis thaliana and isolated two mutants. We showed that the Arabidopsis Ski8 homolog was required for normal plant development and growth, suggesting a conserved somatic function, but that it was not required for meiotic recombination or progression. The data presented here provide strong evidence that the meiotic role of Ski8 is not conserved in Arabidopsis and sequence analysis suggests that this may also be the case in a range of other species.
减数分裂重组涉及DNA双链断裂（DSB）的形成和修复。酿酒酵母中DSB形成所需的基因之一Ski8/Rec103很有趣，因为它在细胞质RNA代谢中也发挥作用，而这一功能与DSB形成难以关联。Ski8在减数分裂中的作用在几种真菌中是保守的，但在真菌界之外尚未得到研究。我们在拟南芥中鉴定出Ski8同源物并分离出两个突变体。我们发现拟南芥Ski8同源物是正常植物发育和生长所必需的，表明其具有保守的体细胞功能，但减数分裂重组或进程并不需要它。本文提供的证据有力地表明，Ski8在减数分裂中的作用在拟南芥中并不保守，序列分析表明在一系列其他物种中可能也是如此。
