细胞心肌成形术：临床应用

Cellular cardiomyoplasty: clinical application.

作者信息

Chachques Juan C, Acar Christophe, Herreros Jesus, Trainini Jorge C, Prosper Felipe, D'Attellis Nicola, Fabiani Jean-Noel, Carpentier Alain F

机构信息

Department of Cardiovascular Surgery, European Hospital Georges Pompidou, University of Paris, Paris, France.

出版信息

Ann Thorac Surg. 2004 Mar;77(3):1121-30. doi: 10.1016/j.athoracsur.2003.09.081.

DOI:10.1016/j.athoracsur.2003.09.081

PMID:14992951

Abstract

Myocardial regeneration can be induced with the implantation of a variety of myogenic and angiogenic cell types. More than 150 patients have been treated with cellular cardiomyoplasty worldwide, 18 patients have been treated by our group. Cellular cardiomyoplasty seems to reduce the size and fibrosis of infarct scars, limit postischemic remodelling, and restore regional myocardial contractility. Techniques for skeletal myoblasts culture and ex vivo expansion using autologous patient serum (obtained from plasmapheresis) have been developed by our group. In this article we propose (1) a total autologous cell culture technique and procedures for cell delivery and (2) a clinical trial with appropriate endpoints structured to determine the efficacy of cellular cardiomyoplasty.

摘要

通过植入多种肌源性和血管生成细胞类型可诱导心肌再生。全球已有150多名患者接受了细胞心肌成形术治疗，我们团队治疗了18名患者。细胞心肌成形术似乎能减小梗死瘢痕的大小并减轻纤维化，限制缺血后重塑，并恢复局部心肌收缩力。我们团队已开发出使用自体患者血清（通过血浆置换获得）进行骨骼肌成肌细胞培养和体外扩增的技术。在本文中，我们提出（1）一种完全自体的细胞培养技术及细胞递送程序，以及（2）一项具有适当终点指标的临床试验，以确定细胞心肌成形术的疗效。