来自患肉瘤的和健康的砂海螂（Mya arenaria L.）血淋巴血清中的不同蛋白质

Different proteins in the hemolymph sera from sarcomatous and healthy soft shell clams, Mya arenaria L.

Sunila I, Dungan C F

Maryland Department of Natural Resources, Cooperative Oxford Laboratory, MD 21654.

Comp Biochem Physiol B. 1992 Jul;102(3):621-5. doi: 10.1016/0305-0491(92)90057-x.

Abstract

Serum proteins showed quantitative and qualitative differences between sarcomatous and healthy soft shell clams, Mya arenaria L. 2. Total protein concentration was significantly higher in the serum of sarcomatous clams than of healthy clams. 3. According to SDS-PAGE, more serum proteins with more variability distinguished sarcomatous clams from healthy ones. 4. Sarcomatous clams had unique serum proteins of M(r)23,000, 45,000 and 54,000. Healthy clams had unique serum proteins of M(r) 24,000, 103,000 and 105,000. 5. During disease progression, sarcoma-specific proteins appeared while normal proteins disappeared. 6. We propose that some sarcoma-associated proteins may have tumor promoting and/or cytotoxic functions and that some normal proteins which disappear during disease progression may be involved in the humoral defense system.

摘要

肉瘤化的软壳蛤（砂海螂）与健康软壳蛤的血清蛋白在数量和质量上存在差异。2. 肉瘤化蛤血清中的总蛋白浓度显著高于健康蛤。3. 根据十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE），更多具有更多变异性的血清蛋白区分了肉瘤化蛤与健康蛤。4. 肉瘤化蛤具有分子量为23,000、45,000和54,000的独特血清蛋白。健康蛤具有分子量为24,000、103,000和105,000的独特血清蛋白。5. 在疾病进展过程中，肉瘤特异性蛋白出现而正常蛋白消失。6. 我们提出，一些肉瘤相关蛋白可能具有促进肿瘤和/或细胞毒性功能，并且一些在疾病进展过程中消失的正常蛋白可能参与体液防御系统。