双壳贝类砂海螂的白血病细胞特异性蛋白。

Leukemia cell specific protein of the bivalve mollusc Mya arenaria.

作者信息

Miosky D L, Smolowitz R M, Reinisch C L

出版信息

J Invertebr Pathol. 1989 Jan;53(1):32-40. doi: 10.1016/0022-2011(89)90071-2.

DOI:10.1016/0022-2011(89)90071-2

Abstract

Soft shell clams, Mya arenaria, develop leukemias in the hemolymph which are fatal. Tissue sections and hemolymph samples from normal and tumor-bearing clams were tested with an anti-leukemic cell specific monoclonal antibody (Mab) "IEII." Evaluation of leukemic cells and normal hemocytes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analyses showed that Mab IEII bound to a large protein of approximately 200 kDa from the tumor cell, but not from the normal cell preparation.

摘要

软壳蛤（Mya arenaria）在血淋巴中会患上致命的白血病。用抗白血病细胞特异性单克隆抗体（Mab）“IEII”对正常蛤和患肿瘤蛤的组织切片及血淋巴样本进行检测。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质免疫印迹分析对白血病细胞和正常血细胞进行评估，结果显示Mab IEII与肿瘤细胞中一种约200 kDa的大蛋白结合，但不与正常细胞提取物中的该蛋白结合。