Breierová E, Kocková-Kratochvílová A
Czechoslovak Collection of Yeasts, Slovak Academy of Sciences, Bratislava.
Cryobiology. 1992 Jun;29(3):385-90. doi: 10.1016/0011-2240(92)90039-5.
Eighteen yeast strains were tested for their ability to survive the freeze-thaw process while being cryoprotected. Cryoprotection was accomplished by combining penetrating and nonpenetrating cryoagents. Four nonpenetrating (two extracellular polysaccharides of yeast and two extracellular glycoproteins of yeast) and two penetrating agents were used together with the nutritive-rich medium. Eight different mixtures were tested. The highest survival rate was obtained with glycoproteins of Rhodosporidium toruloides together with DMSO and nutritive-rich medium.
对18株酵母菌株在冷冻保护剂存在的情况下经历冻融过程的存活能力进行了测试。冷冻保护是通过将渗透性和非渗透性冷冻保护剂组合来实现的。四种非渗透性物质（两种酵母细胞外多糖和两种酵母细胞外糖蛋白）和两种渗透性物质与营养丰富的培养基一起使用。测试了八种不同的混合物。红酵母糖蛋白与二甲基亚砜和营养丰富的培养基一起使用时获得了最高存活率。
