Breierová E
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská, Bratislava, Slovakia.
Lett Appl Microbiol. 1997 Oct;25(4):254-6. doi: 10.1046/j.1472-765x.1997.00215.x.
Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. The glycoprotein secreted by the strain Dipodascus australiensis into the growth medium containing NaCl (8% w/v) was found to be the most effective cryoadditive. It was possible to use this glycoprotein alone (without penetrating agent DMSO) for the cryoprotection of the yeasts studied.
从三种酵母菌种在渗透平衡和不平衡环境中制备了六种细胞外酵母糖蛋白，并将其用作非渗透性低温保护剂。发现澳大利亚双足囊菌菌株分泌到含氯化钠（8% w/v）的生长培养基中的糖蛋白是最有效的低温保护剂。单独使用这种糖蛋白（不添加渗透剂二甲基亚砜）就可以对所研究的酵母进行冷冻保护。
