阿根廷终末期慢性恰加斯心脏病患者心脏病变中的克氏锥虫DNA
Trypanosoma cruzi DNA in cardiac lesions of Argentinean patients with end-stage chronic chagas heart disease.
作者信息
Schijman Alejandro G, Vigliano Carlos A, Viotti Rodolfo J, Burgos Juan M, Brandariz Silvia, Lococo Bruno E, Leze Maria I, Armenti Hector A, Levin Mariano J
机构信息
Laboratorio de Biología Molecular de la Enfermedad de Chagas, INGEBI, Consejo Nacional de Ciencia y Tecnologia, Department of Fisiology, Celular and Molecular Biology, F. CEyN, University of Buenos Aires, Buenos Aires, Argentina.
出版信息
Am J Trop Med Hyg. 2004 Feb;70(2):210-20.
The extent of inflammation, fibrosis, and progression of chronic Chagas heart disease (cChHD) was associated with persistence of parasite DNA in cardiac lesions of necropsies or explants from Argentinean cChHD patients. A Trypanosoma cruzi-based polymerase chain reaction showed a positive result in 1) 15% of cardiac sections with less than 10 mononuclear inflammatory cells/high-power field (440x) (MNC/HPF), 89% with 10-19 MNC/HPF, and 100% with more than 20 MNC/HPF (P < 0.0001); 2) 33% with less than 10% fibrosis, 79% with 10-19% fibrosis, and 100% with more than 20% fibrosis (P < 0.01); 3) 25% of specimens from patients classified in Kuschnir groups 0 and I, 70% in group II and 90% in group III (P < .001); and 4) 45% and 90% of the specimens from cChHD patients without or with heart failure, respectively (P < 0.01). These findings stress the role of the parasite in pathogenesis and disease progression of cChHD.
慢性恰加斯心脏病（cChHD）的炎症、纤维化程度及病情进展与来自阿根廷cChHD患者尸检或心脏外植体心脏病变中寄生虫DNA的持续存在有关。基于克氏锥虫的聚合酶链反应显示：1）在每高倍视野（440倍）单核炎性细胞少于10个（MNC/HPF）的心脏切片中，阳性率为15%；10 - 19个MNC/HPF的切片中，阳性率为89%；超过20个MNC/HPF的切片中，阳性率为100%（P < 0.0001）；2）纤维化少于10%的标本中，阳性率为33%；10 - 19%纤维化的标本中，阳性率为79%；超过20%纤维化的标本中，阳性率为100%（P < 0.01）；3）Kuschnir分组0和I组患者的标本中，阳性率为25%；II组为70%；III组为90%（P < 0.001）；4）cChHD无心力衰竭患者和有心力衰竭患者的标本中，阳性率分别为45%和90%（P < 0.01）。这些发现强调了寄生虫在cChHD发病机制和疾病进展中的作用。
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