Mah James, Prasad Neelanjani
Division of Craniofacial Sciences and Therapeutics, University of Southern California, Los Angeles, California 90089-0641, USA.
Eur J Orthod. 2004 Feb;26(1):25-30. doi: 10.1093/ejo/26.1.25.
External apical root resorption is a common, yet unexplained, phenomenon associated with orthodontic treatment. Available methods of clinical evaluation are radiographic. Biochemical assays offer the advantage of being non-invasive, as well as being diagnostic and potentially prognostic. The hypotheses are firstly that during the process of root resorption, organic matrix proteins are released into the gingival crevicular fluid (GCF) and, secondly, that there is a difference in the levels of these proteins between a group of patients with mild root resorption and a control group. GCF was collected from the permanent central incisors of untreated subjects (controls, n = 20), primary second molars with half of the root resorbed (primary group and positive controls, n = 20) and permanent central incisors with mild root resorption in patients undergoing active orthodontic treatment (orthodontic group, n = 20). Dentine phosphoproteins (DPP) were measured in the GCF using an enzyme-linked immunosorbent assay developed with DPP isolated from human first premolars and an antibody against rat incisor DPP. The primary group showed the highest levels of DPP in the GCF compared with the orthodontic (P = 0.296) and control (P = 0.001) groups. The orthodontic group showed elevated levels relative to the control group (P = 0.046). It is concluded that root resorption can be studied using a biochemical immunoassay and that this method can provide quantitative measurement of DPP in GCF.
根尖外吸收是一种与正畸治疗相关的常见但原因不明的现象。现有的临床评估方法是影像学检查。生化检测具有非侵入性的优势，同时具有诊断和潜在的预后评估作用。其假设一是在牙根吸收过程中，有机基质蛋白释放到龈沟液（GCF）中，二是轻度牙根吸收患者组与对照组之间这些蛋白的水平存在差异。从未经治疗的受试者（对照组，n = 20）的恒中切牙、牙根吸收一半的乳第二磨牙（乳牙组和阳性对照组，n = 20）以及正在接受正畸治疗的患者的轻度牙根吸收的恒中切牙（正畸组，n = 20）中收集龈沟液。使用从人第一前磨牙分离的牙本质磷蛋白（DPP）和抗大鼠切牙DPP抗体开发的酶联免疫吸附测定法测量龈沟液中的牙本质磷蛋白（DPP）。与正畸组（P = 0.296）和对照组（P = 0.001）相比，乳牙组龈沟液中DPP水平最高。正畸组相对于对照组水平升高（P = 0.046）。得出的结论是，可以使用生化免疫测定法研究牙根吸收，并且该方法可以对龈沟液中的DPP进行定量测量。
