Balducci Laura, Ramachandran Amsaveni, Hao Jianjun, Narayanan Karthikeyan, Evans Carla, George Anne
Department of Orthodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA.
Arch Oral Biol. 2007 Mar;52(3):203-8. doi: 10.1016/j.archoralbio.2006.08.018. Epub 2006 Nov 13.
External apical root resorption is a pathologic consequence of orthodontic tooth movement. Cementum and dentin are removed from the root surface while active force is present.
The aim of this study was to identify and quantify extracellular matrix proteins, dentin matrix protein 1 (DMP1), dentin phosphophoryn (PP), and dentin sialoprotein (DSP) in the gingival crevicular fluid (GCF) of subjects undergoing orthodontic treatment.
Subjects with mild (less than 2mm) and severe (more than 2mm) root resorption during orthodontic treatment were identified by radiographs. A control group of subjects with neither signs of root loss nor undergoing orthodontic treatment was also identified. GCF was collected from the upper incisors by using filter paper strips (Periopaper). The absorbed GCF was eluted and the proteins were separated by SDS-PAGE analysis and stained. Western blot and ELISA were also performed. One-way ANOVA and Scheffé test were used for statistical analysis.
SDS-PAGE analysis identified proteins at 77, 66, 55, 50 and 26kDa. Immunoblotting did not show any differential expression pattern between control and study groups. ELISA results revealed a significant difference in the concentrations of DMP1, PP and DSP between control and root resorption groups. Concentration of PP and DSP in severe root resorption group was also statistically higher than in mild root resorption group.
DSP and PP could be suitable biological markers for monitoring root resorption during orthodontic treatment, since a significant difference in the level of these dentin specific proteins is detected in all groups.
根尖外吸收是正畸牙齿移动的病理后果。在施加正畸力时，牙骨质和牙本质会从牙根表面被去除。
本研究旨在鉴定和定量正畸治疗患者龈沟液（GCF）中的细胞外基质蛋白、牙本质基质蛋白1（DMP1）、牙本质磷蛋白（PP）和牙本质涎蛋白（DSP）。
通过X线片确定正畸治疗期间有轻度（小于2mm）和重度（大于2mm）牙根吸收的患者。还确定了一组既无牙根吸收迹象也未接受正畸治疗的对照患者。使用滤纸条（Periopaper）从上门牙收集龈沟液。洗脱吸收的龈沟液，通过SDS-PAGE分析分离蛋白质并进行染色。还进行了蛋白质印迹和酶联免疫吸附测定。采用单因素方差分析和谢费检验进行统计分析。
SDS-PAGE分析鉴定出77、66、55、50和26kDa的蛋白质。免疫印迹未显示对照组和研究组之间有任何差异表达模式。酶联免疫吸附测定结果显示，对照组和牙根吸收组之间DMP1、PP和DSP的浓度有显著差异。重度牙根吸收组中PP和DSP的浓度在统计学上也高于轻度牙根吸收组。
DSP和PP可能是监测正畸治疗期间牙根吸收的合适生物学标志物，因为在所有组中均检测到这些牙本质特异性蛋白水平有显著差异。
