Department of Conservative Dentistry & Endodontics, Centre for Dental Education & Research, All India Institute of Medical Sciences, New Delhi, India.
Int Endod J. 2013 Apr;46(4):371-8. doi: 10.1111/iej.12001. Epub 2012 Oct 24.
To detect and quantify dentine sialoprotein (DSP) in the gingival crevicular fluid (GCF) of luxated teeth.
Eighteen subjects were enroled and distributed as follows. Group I (n = 6, positive control): subjects with primary second molar teeth undergoing physiological root resorption. Group II (n = 6, negative control): subjects with permanent mature maxillary central incisors. Subjects with a recent history (<1 week) of luxation injury were included in group III (n = 6, test group) and standardized digital radiographs with a superimposed mesh gauge were exposed at various time intervals. Percentage of radiographic root resorption (%RRR) was calculated. GCF was collected using microcapillary pipettes. DSP in the GCF was quantified using enzyme-linked immunosorbant assay. Group III was subjected to Spearman's rank test to establish the correlation between the concentration of DSP and %RRR at 6 weeks, 3 and 6 months.
Quantifiable amounts of DSP were released in the GCF of subjects in Group I and III. However, the protein was not detected in Group II. Detectable quantities of DSP were observed in the GCF of luxated teeth before any radiographic evidence of root resorption (base line radiograph). A positive correlation was established at 6 weeks (r = 0.795), 3 (r = 0.755) and 6 month (r = 0.837) between the release of DSP and %RRR (P < 0.05).
Dentine sialoprotein was released in the GCF of luxated teeth and its concentration correlated with the active and remission phases of this pathological process. Further investigation is required to establish a potentially noninvasive aid for diagnosing and monitoring root resorption.
检测并定量分析脱位牙牙龈沟液（GCF）中的牙本质涎磷蛋白（DSP）。
共纳入 18 名受试者，并进行如下分组。I 组（n=6，阳性对照组）：生理性牙根吸收的原发性第二磨牙。II 组（n=6，阴性对照组）：成熟恒上颌中切牙。近 1 周内有脱位损伤史的受试者纳入 III 组（n=6，实验组），并于不同时间点拍摄叠加网格标尺的标准数字化射线照片。计算放射根吸收百分比（%RRR）。采用微量毛细管吸液管收集 GCF，酶联免疫吸附法检测 GCF 中的 DSP。对 III 组进行 Spearman 秩检验，以建立 6 周、3 个月和 6 个月时 DSP 浓度与%RRR 之间的相关性。
I 组和 III 组受试者的 GCF 中释放出可定量的 DSP，但 II 组未检测到该蛋白。在出现放射根吸收（基线射线照片）的任何影像学证据之前，就已在脱位牙的 GCF 中观察到可检测量的 DSP。在 6 周（r=0.795）、3 个月（r=0.755）和 6 个月（r=0.837）时，DSP 的释放与%RRR 之间均建立了正相关（P<0.05）。
DSP 从脱位牙的 GCF 中释放出来，其浓度与该病理过程的活动期和缓解期相关。需要进一步研究以确定其是否可作为一种潜在的非侵入性辅助方法，用于诊断和监测牙根吸收。
