Kato Hideo, Iwata Toshio, Katsu Yoshinao, Watanabe Hajime, Ohta Yasuhiko, Iguchi Taisen
Nihon Bioresearch Inc., 6-104 Majima, Fukuju-cho, Hashima 501-6251, Japan.
J Agric Food Chem. 2004 Mar 10;52(5):1410-4. doi: 10.1021/jf034896d.
We used a modified yeast-based human estrogen receptor alpha (ER alpha) bioassay to determine the estrogenic activity in 22 kinds of diets for experimental animals. The estrogenic activity of each diet was reevaluated by comparison with a calibration curve of 17 beta-estradiol. Almost all of the diets had estrogenic activity. The diets for rabbits and guinea pigs had the highest estrogenic activity compared to any other diets, including those for rats and mice. Estrogenic activity was found in dried skim milk, fishmeal, soybean meal, and alfalfa meal. In the NIH-07 diet opened for the ingredients, estrogenic activity was nearly all derived from the alfalfa meal. Multiple assays were performed to evaluate potential seasonal variations in the estrogenic potency in the raw materials of the rat and mouse diets. We found that the estrogenic activity in these raw materials changed throughout the year.
我们使用改良的基于酵母的人雌激素受体α（ERα）生物测定法来测定22种实验动物饮食中的雌激素活性。通过与17β-雌二醇的校准曲线比较，重新评估了每种饮食的雌激素活性。几乎所有的饮食都具有雌激素活性。与包括大鼠和小鼠饮食在内的任何其他饮食相比，兔子和豚鼠的饮食具有最高的雌激素活性。在脱脂奶粉、鱼粉、豆粕和苜蓿粉中发现了雌激素活性。在公开成分的NIH-07饮食中，雌激素活性几乎全部来自苜蓿粉。进行了多次测定以评估大鼠和小鼠饮食原料中雌激素效力的潜在季节性变化。我们发现这些原料中的雌激素活性全年都在变化。
