嗜热脂肪芽孢杆菌中的质粒维持具有菌株依赖性。

Plasmid maintenance in Bacillus stearothermophilus is strain-dependent.

作者信息

Oskam L, Venema G, Bron S

机构信息

Department of Genetics, Centre of Biological Sciences, Haren, Netherlands.

出版信息

FEMS Microbiol Lett. 1992 Jun 15;72(3):203-8. doi: 10.1016/0378-1097(92)90463-x.

DOI:10.1016/0378-1097(92)90463-x

PMID:1499984

Abstract

We studied the segregational stability of plasmids based on pTB913, a 4.5-kb rolling-circle plasmid derived from the thermophilic Bacillus plasmid pTB19. In Bacillus stearothermophilus the stability of pTB913 derivatives appeared to be strain-dependent. In strain CU21 large amounts of single-stranded pTB913 DNA were found and the plasmid was highly unstable at 57 degrees C. In strain NUB3621, however, very low amounts of single-stranded plasmid DNA were formed and pTB913-based replicons were only slightly unstable at 57 degrees C. The NUB3621/pTB913 host-vector system seems appropriate for molecular cloning. A RepA-based replicon, also derived from pTB19 but replicating by a theta mechanism, was highly unstable in B. stearothermophilus NUB3621.

摘要

我们基于pTB913研究了质粒的分离稳定性，pTB913是一种4.5kb的滚环质粒，源自嗜热芽孢杆菌质粒pTB19。在嗜热脂肪芽孢杆菌中，pTB913衍生物的稳定性似乎取决于菌株。在CU21菌株中发现了大量单链pTB913 DNA，并且该质粒在57℃时高度不稳定。然而，在NUB3621菌株中，仅形成了极少量的单链质粒DNA，并且基于pTB913的复制子在57℃时仅略微不稳定。NUB3621/pTB913宿主-载体系统似乎适用于分子克隆。一种同样源自pTB19但通过θ机制复制的基于RepA的复制子，在嗜热脂肪芽孢杆菌NUB3621中高度不稳定。