用于嗜热脂肪芽孢杆菌克隆的新型穿梭载体。
New shuttle vector for cloning in Bacillus stearothermophilus.
作者信息
De Rossi E, Brigidi P, Welker N E, Riccardi G, Matteuzzi D
机构信息
Department of Genetics and Microbiology A. Buzzati Traverso, University of Pavia, Italy.
出版信息
Res Microbiol. 1994 Oct;145(8):579-83. doi: 10.1016/0923-2508(94)90074-4.
Cloning vector plasmid pRP9 was constructed on the basis of the broad host-range plasmid pLM6. pRP9 was a small plasmid (2.9 kb), possessed a convenient polyrestriction site sequence and efficiently transformed Bacillus subtilis, Bacillus stearothermophilus and Escherichia coli. Furthermore, pRP9 presented a very high segregational stability in Bacillus hosts. Also, the structural stability in Bacillus strains, grown under selective pressure, of pRP9 carrying a 3-kb fragment, was high. No single-stranded and high-molecular weight pRP9 DNA was found in B. stearothermophilus. The host/vector systems described possessed all the properties required for efficient gene cloning.
克隆载体质粒pRP9是在广宿主范围质粒pLM6的基础上构建的。pRP9是一种小质粒（2.9 kb），具有方便的多限制酶切位点序列，能高效转化枯草芽孢杆菌、嗜热脂肪芽孢杆菌和大肠杆菌。此外，pRP9在芽孢杆菌宿主中表现出非常高的分离稳定性。而且，在选择性压力下生长的芽孢杆菌菌株中，携带3 kb片段的pRP9的结构稳定性也很高。在嗜热脂肪芽孢杆菌中未发现单链和高分子量的pRP9 DNA。所描述的宿主/载体系统具备高效基因克隆所需的所有特性。
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