Imanaka T, Ano T, Fujii M, Aiba S
J Gen Microbiol. 1984 Jun;130(6):1399-408. doi: 10.1099/00221287-130-6-1399.
Two different replication determinants were found on an antibiotic resistance plasmid, pTB19, from a thermophilic bacillus. One replication determinant (designated RepA) was functional only in Bacillus subtilis, whereas the other (designated RepB) functioned in both B. subtilis and Bacillus stearothermophilus. A deletion plasmid, pTB90, carrying the RepB derived from pTB19 coincidentally contained the specific 1.0 MDal EcoRI fragment of a cryptic plasmid pBSO2 from B. stearothermophilus. The presence of this 1.0 MDal EcoRI fragment in various deletion plasmids from pTB90 increased transformation frequencies for B. stearothermophilus 10(3) to 10(4) times and lowered plasmid copy numbers in the host strain to about one-tenth of those found for plasmids lacking this fragment.
在一株嗜热芽孢杆菌的抗性质粒pTB19上发现了两种不同的复制决定子。一种复制决定子（命名为RepA）仅在枯草芽孢杆菌中起作用，而另一种（命名为RepB）在枯草芽孢杆菌和嗜热脂肪芽孢杆菌中均起作用。携带源自pTB19的RepB的缺失质粒pTB90恰好包含来自嗜热脂肪芽孢杆菌的隐蔽质粒pBSO2的特定1.0 MDal EcoRI片段。该1.0 MDal EcoRI片段存在于pTB90的各种缺失质粒中，使嗜热脂肪芽孢杆菌的转化频率提高了10³至10⁴倍，并使宿主菌株中的质粒拷贝数降低至缺乏该片段的质粒的约十分之一。
