Chen Shu-an, Zhao Bing, Wang Xiaodong, Yuan Xiaofan, Wang Yuchun
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
Biotechnol Lett. 2004 Jan;26(1):27-30. doi: 10.1023/b:bile.0000009455.31817.a5.
La3+ and Ce3+, either singly or a mixture, promoted crocin production of Crocus sativus callus but Nd3+ had little effect and all metal ions were toxic above 100 microM. La3+ (60 microM) promoted growth of callus significantly but increased crocin only slightly. Ce3+ (40 microM) significantly promoted crocin production but had little effect on cell growth. La3+ (60 microM) and Ce3+ (20 microM) together gave the highest dry weight biomass (20.4 g l(-1)), crocin content (4.4 mg g(-1)) and crocin production (90 mg l(-1)) which were, respectively, 1.7-fold, 4.2-fold and 7.1-fold of those without additions. Nd3+ (40 microM) only slightly promoted cell growth and crocin production.
镧离子（La3+）和铈离子（Ce3+）单独或混合使用时，均可促进藏红花愈伤组织中藏红花素的产生，但钕离子（Nd3+）几乎没有效果，且所有金属离子在浓度高于100微摩尔时均具有毒性。镧离子（60微摩尔）能显著促进愈伤组织生长，但藏红花素增加量较少。铈离子（40微摩尔）能显著促进藏红花素的产生，但对细胞生长影响较小。镧离子（60微摩尔）和铈离子（20微摩尔）共同作用时，干重生物量（20.4克/升）、藏红花素含量（4.4毫克/克）和藏红花素产量（90毫克/升）最高，分别是未添加时的1.7倍、4.2倍和7.1倍。钕离子（40微摩尔）仅略微促进细胞生长和藏红花素的产生。
