Chen Shu-an, Wang Xiaodong, Zhao Bing, Yuan Xiaofan, Wang Yuchun
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, PR China.
Biotechnol Lett. 2003 Aug;25(15):1235-8. doi: 10.1023/a:1025036729160.
Saffron callus was grown in a two-stage culture on B5 medium supplemented with casein hydrolysate (300 mg l(-1)) at 22 degrees C in dark with naphthalene acetic acid (2 mg l(-1)) and 6-benzyladenine (1 mg l(-1)) to give maximum biomass (16 g dry wt l(-1)), and with indole 3-acetic acid (2 mg l(-1)) and 6-benzyladenine (0.5 mg l(-1)) for crocin formation. The maximum crocin production (0.43 g l(-1)) was achieved by this two-stage culture method, which was three times that by a one-stage method.
藏红花愈伤组织在添加了水解酪蛋白（300 mg l(-1)）的B5培养基上进行两阶段培养，于22℃黑暗条件下，添加萘乙酸（2 mg l(-1)）和6-苄基腺嘌呤（1 mg l(-1)）以获得最大生物量（16 g干重l(-1)），添加吲哚-3-乙酸（2 mg l(-1)）和6-苄基腺嘌呤（0.5 mg l(-1)）以促进藏红花素的形成。通过这种两阶段培养方法实现了最大藏红花素产量（0.43 g l(-1)），是单阶段培养方法产量的三倍。
