诺卡氏菌属羧酸还原酶：一个新的醛氧化还原酶家族的克隆、表达及特性分析
Nocardia sp. carboxylic acid reductase: cloning, expression, and characterization of a new aldehyde oxidoreductase family.
作者信息
He Aimin, Li Tao, Daniels Lacy, Fotheringham Ian, Rosazza John P N
机构信息
Division of Medicinal and Natural Products Chemistry and Center for Biocatalysis and Bioprocessing, College of Pharmacy, University of Iowa, Iowa City, Iowa 52242, USA.
出版信息
Appl Environ Microbiol. 2004 Mar;70(3):1874-81. doi: 10.1128/AEM.70.3.1874-1881.2004.
Abstract
We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had K(m) values for benzoate, ATP, and NADPH that were similar to those for natural CAR, and recombinant CAR reduced benzoic, vanillic, and ferulic acids to their corresponding aldehydes. car is the first example of a new gene family encoding oxidoreductases with remote acyl adenylation and reductase sites.
摘要
我们已经克隆、测序并表达了来自诺卡氏菌属的一种独特的依赖ATP和NADPH的羧酸还原酶（CAR）的基因，该酶可将羧酸还原为其相应的醛。含有N端组氨酸亲和标签的重组CAR对苯甲酸、ATP和NADPH的K(m)值与天然CAR相似，并且重组CAR可将苯甲酸、香草酸和阿魏酸还原为其相应的醛。car是编码具有远程酰基腺苷化和还原酶位点的氧化还原酶的新基因家族的首个实例。
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