蛋白质和DNA的超灵敏电化学生物传感：碳纳米管衍生的识别和转导事件放大
Ultrasensitive electrical biosensing of proteins and DNA: carbon-nanotube derived amplification of the recognition and transduction events.
作者信息
Wang Joseph, Liu Guodong, Jan M Rasul
机构信息
Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces, New Mexico 88003, USA.
出版信息
J Am Chem Soc. 2004 Mar 17;126(10):3010-1. doi: 10.1021/ja031723w.
A new strategy for dramatically amplifying enzyme-linked electrical detection of proteins and DNA using carbon nanotubes (CNTs) for carrying numerous enzyme tracers and accumulating the enzymatically liberated product on CNT-modified transducer is described. Such a CNT-derived double-step amplification pathway (of both the recognition and transduction events) allows the detection of DNA and proteins down to 1.3 and 160 zmol, respectively, in 25-50 muL samples and indicates great promise for PCR-free DNA analysis. The new protocol is illustrated for monitoring sandwich hybridization and antibody-antigen interactions in connection with alkaline-phosphatase tracers. The DNA-linking of CNTs and particles holds promise also for assembling hybrid nanostructures relevant to molecular electronic devices.
描述了一种新策略，该策略利用碳纳米管（CNT）显著放大蛋白质和DNA的酶联电检测，碳纳米管用于携带大量酶示踪剂，并在碳纳米管修饰的换能器上积累酶促释放的产物。这种源自碳纳米管的双步放大途径（识别和转导事件均如此）能够在25 - 50微升样品中分别检测低至1.3和160飞摩尔的DNA和蛋白质，显示出无需聚合酶链反应（PCR）进行DNA分析的巨大潜力。结合碱性磷酸酶示踪剂，对监测夹心杂交和抗体 - 抗原相互作用的新方案进行了说明。碳纳米管与颗粒的DNA连接对于组装与分子电子器件相关的混合纳米结构也具有前景。
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