Risøen P A, Rønning P, Hegna I K, Kolstø A-B
Department of Microbiology, School of Pharmacy, University of Oslo, Norway.
J Appl Microbiol. 2004;96(4):648-55. doi: 10.1046/j.1365-2672.2003.02139.x.
The aim of this research was to isolate and characterize an antimicrobial substance from the Bacillus cereus type strain ATCC 14579.
A substance with antimicrobial activity was isolated from B. cereus ATCC 14579. The substance was produced during late exponential growth and well into the stationary phase with a maximum 9 h after inoculation. The inhibitory substance was purified by reverse-phase HPLC and shown to be highly active against closely related Bacillus spp. Clinically relevant species such as Staphylococcus aureus and Micrococcus luteus were also inhibited. The substance was characterized as a bacteriocin-like inhibitory substance (BLIS) with a molecular mass of ca 3.4 kDa. The BLIS was very heat stable, and sensitive only to pronase E and proteinase K. Antimicrobial activity was stable and high in the pH range of 2.0-9.0, and relatively unaffected by organic chemicals.
An antimicrobial substance produced by the B. cereus type strain ATCC 14579 was characterized, with a wide spectrum of activity and the potential to be applied as a control agent against pathogenic bacteria.
The present study is the first report of a substance with antimicrobial activity from the B. cereus type strain.
本研究旨在从蜡样芽孢杆菌模式菌株ATCC 14579中分离并鉴定一种抗菌物质。
从蜡样芽孢杆菌ATCC 14579中分离出一种具有抗菌活性的物质。该物质在指数生长后期产生，并在接种后长达9小时的稳定期内持续产生。通过反相高效液相色谱法纯化该抑制物质，结果表明其对密切相关的芽孢杆菌属具有高活性。临床相关菌种如金黄色葡萄球菌和藤黄微球菌也受到抑制。该物质被鉴定为一种类细菌素抑制物质（BLIS），分子量约为3.4 kDa。该BLIS热稳定性非常高，仅对链霉蛋白酶E和蛋白酶K敏感。抗菌活性在pH值2.0 - 9.0范围内稳定且较高，相对不受有机化学物质的影响。
对蜡样芽孢杆菌模式菌株ATCC 14579产生的一种抗菌物质进行了鉴定，其具有广泛的活性，有潜力作为病原菌控制剂应用。
本研究是关于蜡样芽孢杆菌模式菌株产生具有抗菌活性物质的首次报道。
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