地衣芽孢杆菌P40菌株产生类细菌素物质。
Bacteriocin-like substance production by Bacillus licheniformis strain P40.
作者信息
Cladera-Olivera F, Caron G R, Brandelli A
机构信息
Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brasil.
出版信息
Lett Appl Microbiol. 2004;38(4):251-6. doi: 10.1111/j.1472-765x.2004.01478.x.
AIMS
To investigate the production of bacteriocin-like compounds by Bacillus spp. isolated from the Amazon basin.
METHODS AND RESULTS
An antimicrobial substance produced by Bacillus licheniformis strain P40 was inhibitory to a broad range of indicator strains, such as Listeria monocytogenes, Bacillus cereus and clinical isolates of Streptococcus spp. The compound was stable at 100 degrees C, but lost its activity when treated at 121 degrees C/103.5 kPa for 15 min. It was resistant to the proteolytic action of trypsin and papain but sensitive to pronase E and was stable within a wide range of pH (3-11). The substance was bactericidal and bacteriolytic to L. monocytogenes.
CONCLUSIONS
An antibacterial peptide produced by Bacillus licheniformis was characterized, presenting a broad spectrum of activity against pathogenic and spoilage organisms.
SIGNIFICANCE AND IMPACT OF THE STUDY
The identification of a substance active against important pathogens addresses an important aspect of food safety.
目的
研究从亚马逊盆地分离出的芽孢杆菌属细菌产生类细菌素化合物的情况。
方法与结果
地衣芽孢杆菌菌株P40产生的一种抗菌物质对多种指示菌株具有抑制作用，如单核细胞增生李斯特菌、蜡样芽孢杆菌以及链球菌属的临床分离株。该化合物在100℃时稳定，但在121℃/103.5千帕处理15分钟后失去活性。它对胰蛋白酶和木瓜蛋白酶的蛋白水解作用具有抗性，但对链霉蛋白酶E敏感，并且在较宽的pH范围（3 - 11）内稳定。该物质对单核细胞增生李斯特菌具有杀菌和溶菌作用。
结论
对由地衣芽孢杆菌产生的一种抗菌肽进行了特性鉴定，其对致病和腐败微生物具有广泛的活性。
研究的意义与影响
鉴定出一种对重要病原体具有活性的物质，解决了食品安全的一个重要方面。
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