Dppa3 / Pgc7 / Stella是一种母体因子，在小鼠生殖细胞特化过程中并非必需。
Dppa3 / Pgc7 / stella is a maternal factor and is not required for germ cell specification in mice.
作者信息
Bortvin Alex, Goodheart Mary, Liao Michelle, Page David C
机构信息
Howard Hughes Medical Institute, Whitehead Institute, and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.
出版信息
BMC Dev Biol. 2004 Feb 23;4:2. doi: 10.1186/1471-213X-4-2.
BACKGROUND
In mice, germ cells are specified through signalling between layers of cells comprising the primitive embryo. The function of Dppa3 (also known as Pgc7 or stella), a gene expressed in primordial germ cells at the time of their emergence in gastrulating embryos, is unknown, but a recent study has claimed that it plays a central role in germ cell specification.
RESULTS
To test Dppa3's role in germ cell development, we disrupted the gene in mouse embryonic stem cells and generated mutant animals. We were able to obtain viable and fertile Dppa3-deficient animals of both sexes. Examination of embryonic and adult germ cells and gonads in Dppa3-deficient animals did not reveal any defects. However, most embryos derived from Dppa3-deficient oocytes failed to develop normally beyond the four-cell stage.
CONCLUSION
We found that Dppa3 is an important maternal factor in the cleavage stages of mouse embryogenesis. However, it is not required for germ cell specification.
背景
在小鼠中，生殖细胞是通过构成原始胚胎的细胞层之间的信号传导来确定的。Dppa3（也称为Pgc7或stella）是一种在原肠胚形成期出现的原始生殖细胞中表达的基因，其功能尚不清楚，但最近的一项研究声称它在生殖细胞的确定中起核心作用。
结果
为了测试Dppa3在生殖细胞发育中的作用，我们在小鼠胚胎干细胞中破坏了该基因并生成了突变动物。我们获得了两性均存活且可育的Dppa3缺陷动物。对Dppa3缺陷动物的胚胎和成年生殖细胞及性腺进行检查未发现任何缺陷。然而，大多数源自Dppa3缺陷卵母细胞的胚胎在四细胞阶段后无法正常发育。
结论
我们发现Dppa3是小鼠胚胎发生卵裂阶段的一个重要母体因子。然而，它对于生殖细胞的确定并非必需。
Zotero 插件