保护素（CD59）和巨噬细胞在多发性肌炎和皮肌炎中的免疫定位

Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis.

作者信息

Gendek-Kubiak Hanna, Gendek Ewa G

机构信息

Department of Cytophysiology, Histology and Embryology, Medical University of L?ódź, ul. Narutowicza 60, 90-136 pound ódź, Poland.

出版信息

J Neuroimmunol. 2004 Apr;149(1-2):187-94. doi: 10.1016/j.jneuroim.2003.12.023.

DOI:10.1016/j.jneuroim.2003.12.023

PMID:15020079

Abstract

Immunohistochemical stainings for protectin (CD59) and for the macrophage/monocyte antigen were performed in muscle specimens of polymyositis (PM) and dermatomyositis (DM) patients. In control muscles, a granular immunostaining of CD59 at the surface of 10-15% from among muscle fibers and an intense reaction in endothelia of blood vessels were noted. Strong immunostaining appeared inside vacuoles of vacuolar degeneration changes of muscle fibers, and in regenerating muscle fibers in PM and DM. No macrophages were found next to the CD59 positive muscle regions. The results seem to strongly support a role of the complement (C) system and of the factors protecting cells from the C-induced damage in the mechanism of muscle injury in PM and DM. The findings can have treatment implications.

摘要

在多发性肌炎（PM）和皮肌炎（DM）患者的肌肉标本中进行了针对保护素（CD59）和巨噬细胞/单核细胞抗原的免疫组织化学染色。在对照肌肉中，观察到10%-15%的肌纤维表面有CD59的颗粒状免疫染色，血管内皮有强烈反应。在PM和DM的肌纤维空泡变性变化的空泡内以及再生肌纤维中出现强免疫染色。在CD59阳性的肌肉区域旁边未发现巨噬细胞。结果似乎有力地支持了补体（C）系统以及保护细胞免受C诱导损伤的因子在PM和DM肌肉损伤机制中的作用。这些发现可能对治疗有启示。