Gu Hai-Ying, Chen Zhong, Sa Rong-Xiao, Yuan Su-Su, Chen Hong-Yuan, Ding Yi-Tao, Yu Ai-Min
Department of Chemistry, State Key Laboratory of Coordination Chemistry, Institute of Analytical Science, Nanjing University, Nanjing 210093, China.
Biomaterials. 2004 Aug;25(17):3445-51. doi: 10.1016/j.biomaterials.2003.10.048.
Bioartificial liver and hepatocyte transplantation is anticipated to supply a temporary metabolic support for candidates of liver transplantation or for patients with fulminant liver failure. An essential restriction of this form is the inability to acquire an enough amount of hepatocytes. Enhancement of the proliferation and differentiated function of hepatocytes is becoming a pursued target. Here, porcine hepatocytes were successfully immobilized on nano-sized gold colloid particles to construct a "hepatocyte/gold colloid" interface at which hepatocytes can be quickly proliferated. The properties of this resulting interface were characterized and confirmed by scanning electron microscopy and atomic force microscopy. The proliferative mechanism of hepatocytes was also discussed. The proliferated hepatocytes could be applied to the clinic based on their excellent functions for the synthesis of protein, glucose and urea as well as lower lactate dehydrogenase release.
生物人工肝和肝细胞移植有望为肝移植候选者或暴发性肝衰竭患者提供临时代谢支持。这种形式的一个基本限制是无法获得足够数量的肝细胞。增强肝细胞的增殖和分化功能正成为一个追求的目标。在此，猪肝细胞成功固定在纳米尺寸的金胶体颗粒上，构建了一个“肝细胞/金胶体”界面，在该界面上肝细胞可以快速增殖。通过扫描电子显微镜和原子力显微镜对所得界面的性质进行了表征和确认。还讨论了肝细胞的增殖机制。增殖的肝细胞因其在蛋白质、葡萄糖和尿素合成方面的优异功能以及较低的乳酸脱氢酶释放量而可应用于临床。
