De Bartolo L, Morelli S, Rende M, Gordano A, Drioli E
Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, via P. Bucci cubo 17/C, Rende, Cosenza (CS) I-87036, Italy.
Biomaterials. 2004 Aug;25(17):3621-9. doi: 10.1016/j.biomaterials.2003.10.042.
There has been growing interest in innovative materials with physico-chemical properties that provide improved blood/cell compatibility. We propose new polymeric membranes made of modified polyetheretherketone (PEEK-WC) as materials with potential for use in biohybrid devices. PEEK-WC exhibits high chemical, thermal stability and mechanical resistance. Owing to its lack of crystallinity this polymer can be used for preparing membranes with cheap and flexible methods. We compared the properties of PEEK-WC membranes to polyurethane membranes prepared using the same phase inverse technique and commercial membranes. The physico-chemical properties of the membranes were characterised by contact angle measurements. The different parameters acid (gamma+), base (gamma-) and Lifshitz-van der Waals (gammaLW) of the surface free energy were calculated according to Good-van Oss's model. We evaluated the cytocompatibility of PEEK-WC membranes by culturing hepatocytes isolated from rat liver. Cell adhesion and metabolic behaviour in terms of ammonia elimination, urea synthesis and protein synthesis were evaluated during the first days of culture. Liver cells adhered and formed three-dimensional aggregates on the most tested membranes. PEEK-WC membranes promoted hepatocyte adhesion most effectively. Urea synthesis, ammonia elimination and protein synthesis improved significantly when cells adhered to PEEK-WC membrane. The considerable metabolic activities of cells cultured on this membrane confirmed the good structural and physico-chemical properties of the PEEK-WC membrane that could be a promising biomaterial for cell culture in biohybrid devices.
人们对具有改善血液/细胞相容性的物理化学性质的创新材料的兴趣与日俱增。我们提出用改性聚醚醚酮（PEEK-WC）制成的新型聚合物膜作为有潜力用于生物混合装置的材料。PEEK-WC具有高化学稳定性、热稳定性和机械抗性。由于其缺乏结晶性，这种聚合物可用于通过廉价且灵活的方法制备膜。我们将PEEK-WC膜的性能与使用相同相转化技术制备的聚氨酯膜以及商业膜进行了比较。通过接触角测量对膜的物理化学性质进行了表征。根据古德-范奥兹模型计算了表面自由能的不同参数酸（γ+）、碱（γ-）和利夫希茨-范德华（γLW）。我们通过培养从大鼠肝脏分离的肝细胞来评估PEEK-WC膜的细胞相容性。在培养的最初几天评估了细胞在氨消除、尿素合成和蛋白质合成方面的粘附和代谢行为。肝细胞在大多数测试膜上粘附并形成三维聚集体。PEEK-WC膜最有效地促进了肝细胞的粘附。当细胞粘附到PEEK-WC膜上时，尿素合成、氨消除和蛋白质合成显著改善。在这种膜上培养的细胞具有相当大的代谢活性，证实了PEEK-WC膜良好的结构和物理化学性质，这可能是生物混合装置中细胞培养的一种有前途的生物材料。
