Cho C S, Seo S J, Park I K, Kim S H, Kim T H, Hoshiba T, Harada I, Akaike T
School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea.
Biomaterials. 2006 Feb;27(4):576-85. doi: 10.1016/j.biomaterials.2005.06.008. Epub 2005 Aug 8.
Extracellular matrix (ECM) plays important roles in tissue engineering because cellular growth and differentiation, in the two-dimensional cell culture as well as in the three-dimensional space of the developing organism, require ECM with which the cells can interact. Especially, the bioartificial liver-assist device or regeneration of the liver-tissue substitutes for liver tissue engineering requires a suitable ECM for hepatocyte culture because hepatocytes are anchorage-dependent cells and are highly sensitive to the ECM milieu for the maintenance of their viability and differentiated functions. Galactose-carrying synthetic ECMs derived from synthetic polymers and natural polymers bind hepatocytes through a receptor-mediated mechanism, resulting in enhanced hepatocyte functions. Attachment and functions of hepatocytes were affected by physico-chemical properties including ECM geometry as well as the type, density and orientation of galactose. Also, cellular environment, medium composition and dynamic culture system influenced liver-specific functions of hepatocytes beside ECM.
细胞外基质（ECM）在组织工程中发挥着重要作用，因为在二维细胞培养以及发育中的生物体的三维空间中，细胞的生长和分化都需要细胞能够与之相互作用的细胞外基质。特别是，用于肝组织工程的生物人工肝辅助装置或肝组织替代物的再生需要一种适合肝细胞培养的细胞外基质，因为肝细胞是贴壁依赖性细胞，并且对细胞外基质环境高度敏感，以维持其活力和分化功能。源自合成聚合物和天然聚合物的携带半乳糖的合成细胞外基质通过受体介导的机制结合肝细胞，从而增强肝细胞功能。肝细胞的附着和功能受物理化学性质的影响，包括细胞外基质的几何形状以及半乳糖的类型、密度和取向。此外，除了细胞外基质外，细胞环境、培养基组成和动态培养系统也影响肝细胞的肝特异性功能。
