De Bartolo Loredana, Morelli Sabrina, Bader Augustinus, Drioli Enrico
Research Institute on Membranes and Modelling of Chemical Reactors, IRMERC-CNR, c/o University of Calabria, Rende (CS), Italy.
Biomaterials. 2002 Jun;23(12):2485-97. doi: 10.1016/s0142-9612(01)00383-0.
In bioartificial organs using isolated cells, polymeric semipermeable membranes are used as immunoselective barriers as a means for cell oxygenation and also as substrata for adhesion of anchorage-dependent cells. The capacity of the membrane to perform its functions and to provide a cytocompatible support for cell culture depends in particular on its surface properties. In this study we investigated the physico-chemical aspects of the interaction between the membrane and mammalian cells in order to provide guidelines to the selection of cytocompatible membranes. We evaluated the adhesion and metabolic behaviour of isolated liver cells cultured on various polymeric membranes such as those modified by protein adsorption. The physico-chemical properties of the membranes were characterised by contact angle measurements. The different parameters such as acid (gamma+), base (gamma-) and Lifshitz-van der Waals (gammaLW) of the surface free energy were calculated according to Good-van Oss's model. The adsorption of protein modified markedly both contact angle and components of membrane surface tension. In particular, base parameter of surface tension decreased drastically with increased water contact angle. For each investigated membrane we observed that cell adhesion increased with increasing base parameter of membrane surface tension. The absolute value of cell adhesion is higher in the presence of serum proteins adsorbed on the membrane surface, which change the wettability by increasing the base parameter of surface tension. Also, the metabolic functions improve on hydrophilic membranes. Liver cells synthesised urea with a rate that increased with increasing base parameter value of membrane surface tension. The metabolic activity is particularly expressed at high levels when cells were cultured on polycarbonate and cellulose acetate membranes.
在使用分离细胞的生物人工器官中，聚合物半透膜被用作免疫选择性屏障，作为细胞氧合的手段，也作为贴壁依赖性细胞黏附的基质。膜执行其功能并为细胞培养提供细胞相容性支持的能力尤其取决于其表面性质。在本研究中，我们研究了膜与哺乳动物细胞之间相互作用的物理化学方面，以便为选择细胞相容性膜提供指导。我们评估了在各种聚合物膜（如通过蛋白质吸附改性的膜）上培养的分离肝细胞的黏附及代谢行为。通过接触角测量对膜的物理化学性质进行了表征。根据古德 - 范奥兹模型计算了表面自由能的不同参数，如酸（γ+）、碱（γ-）和利夫希茨 - 范德华（γLW）。蛋白质的吸附显著改变了接触角和膜表面张力的成分。特别是，表面张力的碱参数随着水接触角的增加而急剧下降。对于每个研究的膜，我们观察到细胞黏附随着膜表面张力碱参数的增加而增加。当膜表面吸附血清蛋白时，细胞黏附的绝对值更高，血清蛋白通过增加表面张力的碱参数改变了润湿性。此外，亲水性膜上的代谢功能得到改善。肝细胞合成尿素的速率随着膜表面张力碱参数值的增加而增加。当细胞在聚碳酸酯和醋酸纤维素膜上培养时，代谢活性尤其高水平表达。
