Wang Xuanhui, Grogan Shawn P, Rieser Franz, Winkelmann Verena, Maquet Véronique, Berge Martine La, Mainil-Varlet Pierre
Osteoarticular Research, Institute of Pathology, University of Bern, Murtenstrasse 31, Bern 3010, Switzerland.
Biomaterials. 2004 Aug;25(17):3681-8. doi: 10.1016/j.biomaterials.2003.10.102.
Biological restoration of osteochondral defects requires suitable subchondral support material that also allows the induction of hyaline cartilage tissue. Biphasic implants consisting of pre-fabricated neocartilage and an underlying biodegradable osteoconductive base may meet these requirements. Here we explore various candidate biodegradable support materials onto which neo-cartilage was produced in vitro. Porcine chondrocytes were seeded in a closed and static bioreactor with a base of biomaterial consisting of either poly-L-lactide [P(L)LA], poly-d,l-lactide [P(D,L)LA] or Collagen-hydroxyapatite [Col-HA] and were cultured for 15 weeks. Viable neo-cartilage was produced on each biomaterial with differing amounts of cellular colonisation. P(D,L)LA breakdown was more rapid and uneven among the three biomaterials, leading to constructs of irregular shape. Little or no breakdown or chondrocyte colonisation was evident in P(L)LA. Col-HA constructs were superior in terms of viability, implant morphology and integration between neo-cartilage and biomaterial. These results indicate that our reported system has potential for producing biphasic implants that may be adequate for the repair of osteochondral defects.
骨软骨缺损的生物修复需要合适的软骨下支撑材料，该材料还需能够诱导透明软骨组织的形成。由预制的新软骨和下层可生物降解的骨传导性基底组成的双相植入物可能满足这些要求。在此，我们探索了各种候选的可生物降解支撑材料，在这些材料上体外生成了新软骨。将猪软骨细胞接种到一个封闭的静态生物反应器中，该反应器的基底是由聚-L-丙交酯[P(L)LA]、聚-D,L-丙交酯[P(D,L)LA]或胶原-羟基磷灰石[Col-HA]组成的生物材料，并培养15周。在每种生物材料上都生成了具有不同细胞定植量的活性新软骨。在这三种生物材料中，P(D,L)LA的降解更快且不均匀，导致构建体形状不规则。在P(L)LA中几乎没有降解或软骨细胞定植。Col-HA构建体在活性、植入物形态以及新软骨与生物材料之间的整合方面表现更优。这些结果表明，我们报道的系统具有生产双相植入物的潜力，这些植入物可能足以修复骨软骨缺损。
