用于高通量细胞图案化的喷墨打印

Inkjet printing for high-throughput cell patterning.

作者信息

Roth E A, Xu T, Das M, Gregory C, Hickman J J, Boland T

机构信息

Department of Bioengineering, Rhodes Research Center, Clemson University, 502 Rhodes Hall, Clemson, SC 29634, USA.

出版信息

Biomaterials. 2004 Aug;25(17):3707-15. doi: 10.1016/j.biomaterials.2003.10.052.

DOI:10.1016/j.biomaterials.2003.10.052

PMID:15020146

Abstract

The adaptation of inkjet printing technology to the complex fields of tissue engineering and biomaterial development presents the potential to increase progress in these emerging technologies through the implementation of this high-throughput capability via automated processes to enable precise control and repeatability. In this paper, a method of applying high-throughput inkjet printing to control cellular attachment and proliferation by precise, automated deposition of collagen is presented. The results indicate that commercial inkjet printing technology can be used to create viable cellular patterns with a resolution of 350 microm through the deposition of biologically active proteins. This method demonstrates a combination of off-the-shelf inkjet printing and biomaterials and has potential to be adapted to tissue engineering and colony patterning applications. Adapting this method into the three-dimensional construction of cellular structures for eventual high-throughput tissue engineering using a bottom-up approach is possible.

摘要

将喷墨打印技术应用于组织工程和生物材料开发等复杂领域，通过自动化流程实现这种高通量能力，从而实现精确控制和可重复性，这为推动这些新兴技术的发展带来了潜力。本文介绍了一种通过精确、自动沉积胶原蛋白来应用高通量喷墨打印以控制细胞附着和增殖的方法。结果表明，商业喷墨打印技术可用于通过沉积生物活性蛋白来创建分辨率为350微米的可行细胞图案。该方法展示了现成的喷墨打印技术与生物材料的结合，并且有潜力应用于组织工程和集落图案化应用。采用自下而上的方法将该方法应用于细胞结构的三维构建，最终实现高通量组织工程是可行的。