Manyam Bala V, Dhanasekaran Muralikrishnan, Hare Theodore A
Department of Neurology, Scott & White Clinic and Texas A & M University System Health Science Center College of Medicine, Temple, TX 76508, USA.
Phytother Res. 2004 Feb;18(2):97-101. doi: 10.1002/ptr.1407.
HP-200, which contains Mucuna pruriens endocarp, has been shown to be effective in the treatment of Parkinson's disease. Mucuna pruriens endocarp has also been shown to be more effective compared to synthetic levodopa in an animal model of Parkinson's disease. The present study was designed to elucidate the long-term effect of Mucuna pruriens endocarp in HP-200 on monoaminergic neurotransmitters and its metabolite in various regions of the rat brain. HP-200 at a dose of 2.5, 5.0 or 10.0 g/kg/day was mixed with rat chow and fed daily ad lib to Sprague-Dawley rats (n = 6 for each group) for 52 weeks. Controls (n = 6) received no drug. Random assignment was made for doses and control. The rats were sacrificed at the end of 52 weeks and the neurotransmitters were analyzed in the cortex, hippocampus, substantia nigra and striatum. Oral administration of Mucuna pruriens endocarp in the form of HP-200 had a significant effect on dopamine content in the cortex with no significant effect on levodopa, norepinephrine or dopamine, serotonin, and their metabolites- HVA, DOPAC and 5-HIAA in the nigrostriatal tract. The failure of Mucuna pruriens endocarp to significantly affect dopamine metabolism in the striatonigral tract along with its ability to improve Parkinsonian symptoms in the 6-hydorxydopamine animal model and humans may suggest that its antiparkinson effect may be due to components other than levodopa or that it has an levodopa enhancing effect.
含有刺蒺藜内果皮的HP - 200已被证明对帕金森病的治疗有效。在帕金森病动物模型中，刺蒺藜内果皮也被证明比合成左旋多巴更有效。本研究旨在阐明HP - 200中的刺蒺藜内果皮对大鼠脑不同区域单胺能神经递质及其代谢产物的长期影响。将剂量为2.5、5.0或10.0 g/kg/天的HP - 200与大鼠饲料混合，每日随意喂食给斯普拉格-道利大鼠（每组n = 6），持续52周。对照组（n = 6）不给予药物。对剂量组和对照组进行随机分配。在52周结束时处死大鼠，并对皮质、海马、黑质和纹状体中的神经递质进行分析。以HP - 200形式口服刺蒺藜内果皮对皮质中的多巴胺含量有显著影响，而对黑质纹状体通路中的左旋多巴、去甲肾上腺素或多巴胺、5-羟色胺及其代谢产物——高香草酸、3,4-二羟基苯乙酸和5-羟吲哚乙酸没有显著影响。刺蒺藜内果皮未能显著影响纹状体黑质通路中的多巴胺代谢，同时其在6-羟基多巴胺动物模型和人类中改善帕金森症状的能力可能表明，其抗帕金森作用可能归因于左旋多巴以外的成分，或者它具有增强左旋多巴的作用。
