Marques Márcia M, Pereira Aymann N, Fujihara Neusa A, Nogueira Fernando N, Eduardo Carlos P
Department of Operative Dentistry, School of Dentistry, University of São Paulo, São Paulo 05508-900, Brazil.
Lasers Surg Med. 2004;34(3):260-5. doi: 10.1002/lsm.20008.
Low-power lasers improve wound healing. Cell proliferation and protein secretion are important steps of this process. The aim of this study was to analyze both protein synthesis and ultrastructural morphology of human gingival fibroblasts irradiated by a low-power laser.
STUDY DESIGN/MATERIALS AND METHODS: The cell line FMM1 was grown in nutritional deficit. Laser irradiation was carried out with a gallium-aluminum-arsenate (Ga-Al-As) diode laser (904 nm, 120 mW, energy density of 3 J/cm(2)). The protein synthesis analysis and ultrastructural morphology of control (non-irradiated) and irradiated cultures were obtained.
There were changes in the structure of cytoplasm organelles of treated cells. The procollagen was not altered by the laser irradiation; however, there were a significant reduction of the amount of protein in the DMEM conditioned by irradiated cells.
Low-power laser irradiation causes ultrastructural changes in cultured fibroblasts. We suggest that these alterations may lead to disturbances in the collagen metabolism.
低功率激光可促进伤口愈合。细胞增殖和蛋白质分泌是这一过程的重要步骤。本研究旨在分析低功率激光照射后人牙龈成纤维细胞的蛋白质合成及超微结构形态。
研究设计/材料与方法：细胞系FMM1在营养缺乏条件下培养。使用砷化镓铝（Ga-Al-As）二极管激光（904nm，120mW，能量密度3J/cm²）进行激光照射。对对照（未照射）和照射培养物进行蛋白质合成分析及超微结构形态观察。
处理后细胞的细胞质细胞器结构发生变化。激光照射未改变前胶原；然而，照射细胞条件培养基中的蛋白质量显著减少。
低功率激光照射可导致培养的成纤维细胞超微结构改变。我们认为这些改变可能导致胶原代谢紊乱。
