Pourzarandian Amir, Watanabe Hisashi, Ruwanpura Senarath M P M, Aoki Akira, Ishikawa Isao
Section of Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
J Periodontol. 2005 Feb;76(2):187-93. doi: 10.1902/jop.2005.76.2.187.
Low-level laser irradiation has been reported to enhance wound healing. Activation of gingival fibroblasts (GF) has a potential for early wound healing in periodontal treatment. The present study aimed to investigate the direct effect of low-level Er:YAG laser irradiation on gingival fibroblasts proliferation in order to clarify the laser effect on healing.
Cultured human gingival fibroblasts (hGF) were exposed to low-power, pulsed Er:YAG laser irradiation with different energy densities ranging from 1.68 to 5.0 J/cm(2). The cultures were analyzed by means of trypan blue staining and counted under a light microscope. The effect of Er:YAG laser on hGF was also evaluated using a transmission electron microscope (TEM).
Cultures irradiated with Er:YAG laser presented faster cell growth when compared with untreated controls. This difference was statistically significant. Transmission electron microscopy revealed rough endoplasmic reticulum, prominent Golgi complexes, and mitochondria after laser irradiation.
Our results showed that the low-level Er:YAG laser irradiation stimulates the proliferation of cultured gingival fibroblasts. The optimal stimulative energy density was found to be 3.37 J/cm(2). This result suggests that Er:YAG laser irradiation may be of therapeutic benefit for wound healing.
据报道，低强度激光照射可促进伤口愈合。牙龈成纤维细胞（GF）的激活在牙周治疗中对早期伤口愈合具有潜在作用。本研究旨在探讨低强度铒激光（Er:YAG）照射对牙龈成纤维细胞增殖的直接影响，以阐明激光对愈合的作用。
将培养的人牙龈成纤维细胞（hGF）暴露于能量密度范围为1.68至5.0 J/cm²的低功率脉冲Er:YAG激光照射下。通过台盼蓝染色对培养物进行分析，并在光学显微镜下计数。还使用透射电子显微镜（TEM）评估了Er:YAG激光对hGF的影响。
与未处理的对照组相比，接受Er:YAG激光照射的培养物细胞生长更快。这种差异具有统计学意义。透射电子显微镜显示激光照射后有粗糙内质网、突出的高尔基体复合体和线粒体。
我们的结果表明，低强度Er:YAG激光照射可刺激培养的牙龈成纤维细胞增殖。发现最佳刺激能量密度为3.37 J/cm²。这一结果表明，Er:YAG激光照射可能对伤口愈合具有治疗益处。
