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低强度激光疗法对小鼠成纤维细胞和人成纤维细胞增殖及细胞毒性的影响：一项研究。

Effect of low-level laser therapy on proliferation and cytotoxicity of mouse fibroblasts and human fibroblasts: An study.

作者信息

Patil Mallanagouda B, Pai Deeksha D, Prakash Shobha

机构信息

Department of Periodontics, College of Dental Sciences, Davanagere, Karnataka, India.

出版信息

J Indian Soc Periodontol. 2024 May-Jun;28(3):338-348. doi: 10.4103/jisp.jisp_376_23. Epub 2024 Dec 2.

DOI:10.4103/jisp.jisp_376_23
PMID:39742060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11684573/
Abstract

BACKGROUND

Diode laser is known for its biostimulatory effects on various cell populations such as osteoblasts and fibroblasts. The usage of low-level laser therapy for photobiomodulation depends on its type, emission wavelength, and energy selected. Previously performed studies have determined its effect on cell proliferation and cytotoxicity; however, the results were inconsistent.

OBJECTIVES

The objectives of this study were to evaluate and compare diode laser effect on mouse embryonic fibroblasts (MEFs) and human gingival fibroblasts (HGFs) for proliferation and cytotoxicity.

MATERIALS AND METHODS

32 samples, 16 wells of MEF cells (Group I) and HGF cells (Group II) each with 8 subgroups containing control (no laser irradiation) and experimental (laser irradiated) groups were cultured after being seeded at 4000 cells/ well with 4cm inter-well distance. Experimental group cell cultures were irradiated with a single dose of 810 nm diode laser (energy 1J/cm, 12.5 mW, 80 s/well) using continuous wave mode after 1 day of incubation. Spectrophotometric analysis was done after 24 h of laser irradiation for cell proliferation and cell cytotoxicity on the 2, 3, and 7 days on all groups.

RESULTS

Human and mouse fibroblast groups showed positive results when irradiated with an 810 nm laser. A hundred percent proliferation was seen for MEF in 7 days and HGF in 2 days. Two percent cytotoxicity was seen on the 2 and 3 day in MEF. Increased cell proliferation and minimal cytotoxic effects were seen with diode laser usage.

CONCLUSION

Low-level laser irradiation indicates an increase in cell proliferation with less cytotoxicity on mouse and human fibroblasts.

摘要

背景

二极管激光以其对多种细胞群体（如成骨细胞和成纤维细胞）的生物刺激作用而闻名。低强度激光疗法用于光生物调节取决于其类型、发射波长和所选能量。先前进行的研究已经确定了其对细胞增殖和细胞毒性的影响；然而，结果并不一致。

目的

本研究的目的是评估和比较二极管激光对小鼠胚胎成纤维细胞（MEF）和人牙龈成纤维细胞（HGF）增殖和细胞毒性的影响。

材料和方法

32个样本，每组16孔MEF细胞（I组）和HGF细胞（II组），每组有8个亚组，包含对照（无激光照射）和实验（激光照射）组，以4000个细胞/孔接种，孔间距为4cm，接种后进行培养。孵育1天后，对实验组细胞培养物使用连续波模式，用单剂量810nm二极管激光（能量1J/cm，12.5mW，80s/孔）进行照射。在激光照射24小时后，对所有组在第2、3和7天进行分光光度分析，以检测细胞增殖和细胞毒性。

结果

人源和鼠源成纤维细胞组在接受810nm激光照射时显示出阳性结果。MEF在7天内实现了100%的增殖，HGF在2天内实现了100%的增殖。MEF在第2天和第3天出现了2%的细胞毒性。使用二极管激光可观察到细胞增殖增加且细胞毒性最小。

结论

低强度激光照射表明对小鼠和人成纤维细胞的细胞增殖增加且细胞毒性较小。

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